【摘要】 目的探讨体外培养过程中细胞因子激活的杀伤细胞（CIK）免疫表型的动态变化。方法通过向外周血单个粒细胞（PBMC）中加入白细胞介素2（IL-2）、γIL-1α、γ干扰素（γ-IFN）与抗CD3McAb,诱导出CIK细胞。经流式细胞分析法对CIK细胞进行动态表型分析。结果动态表型分析显示,在第14²1天时CD3+CD16+56+细胞为（31·6±5·5）%^35·8±9·7%,呈高表达的平台期。结论CIK细胞在体外培养14²1d,CIK细胞CD3+CD16+56+双阳性细胞呈高表达,此时临床应用可取得较好疗效。更多还原

【Abstract】 Objective To study the dynamic developments of the immune phenotype of CIK cells in vitro. Methods The CIK cells were induce d by adding IL-2,γIL-1α,γ-IFN and CD3 McAb in vitro.The immune phenotype of the CIK cells was analized by flow cytometry. Results The immune phenotype was analyzed. The CD3⁺CD16+56⁺ of CIK cells was （31.6±5.5）%^35.8±9.7% on the 14t h day to 21st day,which showed a flat roof with high expression. Conclusion The CD3⁺CD16+56⁺ of double positive CIK cells ha s a high expression cultured in vitro on the 14th day to 21st day.A better cu rative effect may be obtained when used clinically during this period of time.更多还原