【摘要】 目的探讨高糖对内皮细胞产生活性氧和转化生长因子β1(TGF β1)的影响,以及茶多酚的干预作用。方法培养人脐静脉血管内皮细胞(ECV304),分为正常对照组、高糖组、茶多酚组和茶多酚干预组。培养0、12、36 h后,用分光光度比色法测定细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,用RT PCR法测定细胞内TGF β1 mRNA的变化。结果高糖导致内皮细胞SOD活性下降和MDA含量升高,上调TGF β1 mRNA表达,其变化随时间延长更为明显。茶多酚干预可拮抗高糖时内皮细胞的上述改变。结论茶多酚有抑制高糖对内皮细胞的损伤作用。更多还原

【Abstract】 Objective To investigate the effect of high glucose on the production of reactive ^oxygen species （ROS） and transforming growth factor β₁ （TGF-β₁） in human umbilical vein endothelial cells（ECV304）,and the intervention of tea polyphenols （TPs）. Methods ECV304 was cultured ⁱn different conditional media and divided into control group, high glucose group, TPs group and TPs treatment group. After 0,12 and 36h, the activity of SOD and the contents of MDA in supernatant were detected by spectrophotometry, meanwhile the mRNA expression of TGF-β₁ mRNA was determined by RT-PCR. Results The activity of SOD was found to be significantly decreased , while the contents of MDA and the expression of TGF-β₁ mRNA increased in high glucose. Such changes ^became more significant as time prolonged.TPs could inhibit all these changes of ECV304 in high ^glucose. Conclusion TPs may diminish the harmful effect of high glucose on the endothelial cells.更多还原