【摘要】 利用粗线期染色体和DNA纤维的荧光原位杂交(FISH)技术分析了水稻广陆矮四号(Oryzasativassp.indicacv.GuangluaiNo.4)的端粒序列。粗线期染色体荧光原位杂交结果表明,大多数染色体的末端都有端粒串联重复,但信号的强度在不同染色体上是不同的。伸展DNA纤维荧光原位杂交结果显示,端粒最长的线状信号长度为6.55μm,最短的为1.82μm,依据2.51kb/μm的标准,它们分别相当于16.44kb和4.56kb。端粒的平均信号长度为3.62±1.32μm,相当于9.09±3.31kb。由此可以估计,最长的、最短的和平均长度的端粒拷贝数约为2349、651和1298±473。更多还原

【Abstract】 Telomere sequences were analyzed by using pachytene chromosome and extended DNA fiber FISH in rice (Oryza,sativa ssp.indica cv.-Guangluai No.4).Pachytene FISH results showed that most of chromosome ends possess the telomere tandem repeats,but the signals on different chromosomes were not the same in intensity.Fiber FISH results indicated that the longest string of beads was 6.55 μm,while the shortest one was 1.82 μm long,which were equal to 16.44 and 4.56 kb correspondingly based on a stretching factor of 2.51 kb/μm.The average value of signal length was 3.62±1.32 μm,i.e.9.09±3.31 kb.It could be estimated that the longest and the shortest as well as the average value were equal to 2 349,651 and 1 298±473 of copy number respectively.更多还原