【摘要】 H9020175是一个通过杂交和回交选育的普通小麦华山新麦草易位系,接种鉴定表明其对条锈病具有优良抗性。遗传学分析证明易位系H9020175的抗条锈性是由单基因控制的显性性状,抗性基因来自于华山新麦草,暂定名为YrHua。为了标记这个来自华山新麦草的抗条锈病基因,利用H9020175与感病小麦品种铭贤169杂交,建立了F2分离群体。应用81对AFLP引物对119个经条锈菌生理小种CY30接种鉴定的F2单株进行了分析,结果得到两个与YrHua基因连锁的AFLP标记PM14(301)和PM42(249),遗传距离分别为5.4cM和2.7cM,并分别位于目标基因的两侧。将标记片段克隆、测序后,根据序列信息和酶切位点多态性设计特异性引物,将AFLP标记PM14(301)转换成了简单的PCR标记。研究结果为标记辅助育种提供了分子选择工具,同时也为进一步精细定位和图位克隆YrHua基因奠定了基础。更多还原

【Abstract】 The H9020-17-5,a common wheat-Psathyrostachys huashanica Keng translocation line,possesses excellent resistance to wheat stripe rust.Genetic analysis of F2 and BC1 populations derived from H9020-17-5 × Mingxian169 indicated that resistance to stripe rust in H9020-17-5 was a dominant character controlling by single gene originated from Ps.huashanica.This resistance gene originated from Ps.huashanica was first reported in the present study and named as YrHua .In order to map the resistance gene YrHua,AFLP approach was employed to analyze the 119 individuals of H9020-17-5 × Mingxian169 F2 population which were inoculated by stripe rust isolate CY30.As a result,two markers,PM14(301) and PM42(249) were found to be linked to the resistance gene YrHua,and the genetic distances between the markers and target gene were 5.4 cM and 2.7 cM,respectively.For the convenience of marker-assisted selection in wheat breeding,one of the two AFLP markers was converted to PCR marker using a pair of special primers based on the DNA sequence of PM14(301) and the polymorphism of restriction site.Our research results provided a useful tool for marker-assisted selection and laid a foundation of fine mapping and map based cloning of YrHua gene.更多还原