【摘要】 目的探讨猫儿眼植物酸对人SGC7901细胞增殖影响及其机制。方法采用流式细胞仪、MTT法和台盼蓝染色法测定猫儿眼植物酸对人SGC7901细胞增殖的影响。结果分别给予猫儿眼植物酸10.0、1.0、0.1mg·L-1培养48h,MTT法测定结果显示,猫儿眼植物酸对人SGC7901细胞增殖的抑制率分别为82.9%、42.7%和24.6%;台盼蓝法计数结果显示,给予猫儿眼植物酸后,活细胞数随培养时间的延长而减少,到48h,对SGC7901细胞增殖抑制率分别为76.4%、56.9%、42.6%,凋亡率分别为26.8%,22.5%和15.6%。结论猫儿眼植物酸对人SGC7901细胞增殖具有明显的抑制作用,其抑制作用强度随给药浓度的增加和作用时间的延长而递增。更多还原

【Abstract】 Aim To study the inhibitory effect of Maoeryan vegetable acid on human SGC-7901 strain cells and the mechamism of it. Methods The inhibitory effects of Maoeryan vegetable acid on human SGC-7901 strain cells were determined by flow cytometry, methods of MTT and trypan-blue.Results Maoeryan vegetable acid at a does of 10.0, 1.0 and 0.1 mg·L -1 was given to human SGC-7901 strain cells for 48 h,respectively, and the inhibitory rate of human SGC-7901 strain cells was 82.9%,42.7% and 24.6%,respectively;cellular growth inhibitory rate was 76.4%,56.9% and 42.6%, respectively;as well as apoptosis rate was 26.8%,22.5% and 15.6%, respectively. Conclusion The Maoeryan vegetable acid has significant inhibitory effect on human SGC-7901 strain cells and the inhibitory effect increases with the increase of concentration and time.更多还原