【摘要】 目的　研究半边旗提取物5F对微管蛋白聚合的影响。方法　在离体条件下,将受试化合物加入到微管蛋白聚合解聚反应体系中,于37℃、350nm下检测反应体系吸光值的变化。结果　5F能抑制反应体系吸光值升高,提示它对微管蛋白聚合具有抑制作用并能抑制其解聚。结论　5F能抑制体外微管蛋白聚合,从而可能是影响细胞的细胞周期及诱导凋亡途径之一。更多还原

【Abstract】 Aim To study the effect of 5F on tubulin polymerization.Methed 5F was added into the tubulin polymerization-depolymerization system and the changes of Absorption values at 350 nm, 37 ℃ were recorded. Results 5F decreased the Absorption values of the reaction system.and markedly inhibited not only tubulin polymerization but also tubulin depolymerization. Conclusion 5F inhibits tubulin polymerization and depolymerization, and leads to cell cycle arrested, and possibly induces apoptosis.更多还原