【摘要】 为了观察吡咯烷二硫代氨基甲酸盐(pyrroledithiocarbomate,PDTC)体外对柔红霉素的化疗增敏作用,利用MTT法观察联用PDTC和柔红霉素处理难治性白血病患者骨髓单个核细胞增殖的变化。结果表明,加入PDTC后柔红霉素对白血病耐药细胞的抑制率增强(P<0.05)。当PDTC的浓度为50μmol/l时柔红霉素对白血病耐药细胞的抑制最明显。结论:PDTC体外能增敏柔红霉素对耐药白血病细胞的抗肿瘤作用,其中以50μmol/L浓度的PDTC为最佳抑制浓度。更多还原

【Abstract】 To investigate the chemosensitizing effect of pyrroledithiocarbomate (PDTC )on daunorubicin in drug-resistant leukemic cells in vitro, MTT method was used to observe the changes of the proliferation of intractable leukemia MNC treated with daunorubicin ( 30 μg/ml) combined with PDTC(25,50 or 100 μmol/L). The results showed that inhibiting rate of daunorubicin combined with PDTC(25,50 or 100 μmol/L) on drug-resistant leukemic cells was significantly higher than that of daunorubicin alone (P<0.05). Among the three different doses of PDTC, the concentration of 50 μmol/L of PDTC inhibited the proliferation of drug-resistant leukemic cells significantly. In conclusion, PDTC can sensitize anti-tumor effect of daunorubicin in vitro. The concentration of 50 μmol/L of PDTC has stronger chemosensitizing effect on daunorubicin than that of the other concentrations of PDTC (25 μmol/L or 100 μmol/L)in vitro.更多还原