【摘要】 目的构建稳定表达Flt3分子的转基因细胞株。方法将Flt3基因重组入逆转录病毒载体pEGZ-term,通过293T细胞的包装获得具有感染能力的完整重组病毒载体,进而采用“一箭双星法”即同时感染BaF和L929细胞,构建Flt3转基因细胞株。结果成功获得稳定表达Flt3的转基因细胞株BaF/Flt3和L/Flt3。结论采用“一箭双星法”成功构建Flt3转基因细胞株,为以Flt3为靶分子的研究奠定了物质基础,也为类似的高难度的转基因细胞株的构建提供了有价值的经验。更多还原

【Abstract】 Objective To construct transgenic cell line that stably expressing Flt3.Methods Flt3 gene was subcloned into retroviral expressing vector pEGZ-term.Whole combinated retroviral expressing vector was integrated by package cell;293T cell,and then Flt3 transgenic cell line was constructed by transfecting BaF Cells and L929 cells at same time.So this new strategy was named "kill two birds with one stone".Results Transgenic cell lines those stably expressing Flt3,including BaF/Flt3 and L/Flt3,were constructed successfully.Conclusion Flt3 transgenic cell lines were established successfully.It provided valuable tools to study Flt3 biological functions.The"kill two birds with one stone"strategy provided an effective solution to the low efficiency of transgenic cell construction of the suspending cell.更多还原