【摘要】 应用循环伏安法(CV)研究了药物丝裂霉素(MC)与小牛胸腺DNA(ctDNA)在自制的浸蜡石墨电极上的相互作用。在Na2HPO4NaH2PO4溶液体系中,MC及MCctDNA反应物在电极上出现良好的还原-氧化峰。根据相应的电化学理论方程,由实验数据求得了MC及MCDNA反应复合物的自由扩散系数及反应的表观速率常数。结果表明,固体浸蜡石墨电极可方便、灵敏地用于研究丝裂霉素及与ctDNA相互作用的电化学特性并能用于求取相关参数。更多还原

【Abstract】 Aim To investigate the electrochemical behaviors of Mitomycin C (MC) and its interaction with calf thymus DNA (ctDNA). Methods The cyclic voltammetry (CV) was carried out at a paraffined graphite electrode. Results MC showed a well-defined oxidation-reduction peak. As a result of reaction with ctDNA, the peak current of MC decreased apparently. According to corresponding electrochemical equations, the diffusion coefficient of both free and MC-DNA complex have been determined, and the heterogeneous rate constants were also obtained simultaneously. Conclusion The solid paraffined graphite electrode could be used to estimate parameters of the interaction between DNA and MC, and provide the convenient and sensitive analysis.更多还原