【摘要】 建立了离子增强反相高效液相色谱法(RP-HPLC)快速测定落羽杉中痕量莽草酸的方法．用色谱柱Hyper- silODS 25μm(4．6 mm×150 mm),以HClO4(pH=2．5)溶液为流动相,柱温30℃时于214 nm波长处以外标法测定．结果表明莽草酸在2．22×10-8-3．59×10-4g／mL范围内,浓度与峰面积有良好的线性关系(r=0．999 8)．莽草酸的回收率为98．52％-98．91％,相对标准偏差为1．45％-1．70％(n=5)．检测限为2．02×10-10g／mL(S／N= 2)．实验表明该法简便、快速,应用于实际落羽杉样品中莽草酸的测定,取得了很好的结果．更多还原

【Abstract】 A accurate and reliable ion-improved reversed-phase HPLC method was established for the determination of rudimental Shikimic acid in Taxodium distichum. Shikimic acid can be separated on Hypersil ODS2 5 μm(4. 6 mm×150 mm) column by using Perchloric acid(pH = 2. 25) as mobile phase with UV detector at a wavelength of 214 nm. The standard curve was linear in the concentration range 2. 22×10-8-3. 59×10-4 g/mL and the correlation coefficient was 0.9998. The recovery and RSD was 98.52%-98.91% and 1.45%-1.70% (n=5) respectively. The detection limit was 2.02×10-10 g/mL(S/N=2). The method has been successfully applied to the determination of rudimental trace Shikimic acid in Taxodi-um distichum, which was proved to be simple and rapid.更多还原