【摘要】 应用荧光光谱技术研究了农药除草剂二氯喹啉酸与牛血清白蛋白的结合反应的热力学特征.研究结果表明:二氯喹啉酸对牛血清白蛋白有较强的荧光猝灭作用,且符合静态猝灭机理.从荧光猝灭光谱数据,由stern-volmer方程、double-reciprocal方程和热力学公式得到了结合反应的结合常数KLB=2.345×102mol.L-1(24℃),KLB=5.071×102mol.L-1(32℃)和热力学参数等.更多还原

【Abstract】 The thermochemical characteristics of binding reaction between agricultural pesticide herbicide quinclorac（QUC） and bovine serum albumin （BSA） was studied by fluorescence spectrosum technology in this text. Studies have shown: quinclorac has a powerful ability to quench the BSA fluorescence intensity via a nonradiative nenergy transfer mechanism, and the fluorescence quenching data was analyzed according to Stern-Volmer equation and double-reciprocal equation, and the binding constant (K_LB=2.345×10² mol·L^-1 and K_LB=5.071×10² mol·L^-1), and the thermodynamic parameters were obtained.更多还原