【摘要】 目的研究甘珀酸钠苦参素包合物急性毒性及对CCl4肝损伤小鼠的保护作用。方法采用序贯法,静脉注射测定药物的LD50。采用CCl4制备小鼠急性肝损伤模型,检测经不同比例甘珀酸钠苦参素包合物干预后小鼠血清谷草转氨酶(GOT)、谷丙转氨酶(GPT),取肝脏组织做HE染色常规光镜观察。结果甘珀酸钠苦参素1∶1、1∶22、∶1包合物的急性毒性均比其前体药物甘珀酸钠、苦参素低;与CCl4模型组比较,经甘珀酸钠苦参素1∶12、∶1包合物处理的小鼠血清GOT、GPT水平较低(P<0.05,或P<0.01);小鼠肝脏组织学检查结果显示,甘珀酸钠苦参素2∶1包合物组肝脏形态有明显改善。结论甘珀酸钠与氧化苦参碱不同比例包合物的毒性比其前体药物低,对小鼠急性CCl4肝损伤有保护作用。更多还原

【Abstract】 Objective To study the acute toxicity of clathrate compound of carbenoxolone and oxymatrine and its protectire effects on injured liver induced by CCl₄ in mice. Methods The Sequence Method was employed to test LD₅₀ of the clathrate compound injected intravenously. The GOT and GPT in serum were measured in acute hepatic injury models by CCl₄ proportionally injected the clathrate compound intramuscularly in mice. The hepatic structures were observed under microscope. Results The acute toxicity of LD₅₀ of the clathrate compound in 1∶1,1∶2 and 2∶1 were lower than that of both carbenoxolone sodium and oxymatrine. Compared with the model group by CCl₄, the GOT and GPT levels in serum were lower in the clathrate compound of 1∶1 and 2∶1（P<0.05, P<0.01）. The clathrate compound in 2:1 presented obvious effects in hepatic morphology. Conclusions[WT5B1] The toxicity of the clathrate compound in different proportion decreased, compared with their pre. The proportion of 2∶1 presents protection from hepatic injury by CCl₄ in mice.更多还原