【摘要】 对云南野生白芨(Bletilla striata)进行组培快繁研究,结果表明:M s+6-BA 1.0~3.0 mg/L+NAA 0.2 mg/L可诱导茎尖及侧芽萌生增殖芽;M s+KT 4.0 mg/L+NAA 0.2 mg/L诱导增殖的效果最好,培养40 d后增殖率可达450%;1/2 M s+NAA 0.5 mg/L有利于生根,且植株长势健壮。炼苗基质以60%腐叶土+30%珍珠岩+10%素红土为佳;炼苗温度23~27℃,空气相对湿度80%~90%有利于提高炼苗成活率。更多还原

【Abstract】 Cell cu lture was m ade of w ild B letilla grow ing in Yunnan province for its rap id propagation.MS+6-BA 1.0 ~3.0 mg /L+ NAA 0.2 mg /L induced proliferation buds from its tip m eristem s and lutred buds.MS+KT 4.0 mg /L+NAA 0.2 mg /L was the best pro-liferation m ed ium,giving a proliferation rate of 450% after 40 days ’ cu lture.1 /2 MS+NAA 0.5 mg /L was favorab le for rooting and the vigorous growth of the induced shoots.The bestm ed ium for the harden ing of the regenerated p lantlets was a compostm ade of humus soil 60%,perlite 30% and simp le red soil 10%.Hardened under a temperature of 23 ~27℃and a relative hum id ity of 80% ~90%,the re-generated p lantlets survived well.更多还原