【摘要】 为进一步研究鸡在出孵1个月左右时间内T、B淋巴细胞及其亚群的发育状况,将SPF雏鸡分别在3、5、7、9、11、14、17、22、27、32日龄时取血液、脾脏、法氏囊及胸腺,分离并制成淋巴细胞悬液,流式细胞仪双染色法检测Bu-1a+B淋巴细胞、CD3+、CD4+、CD8+和TCRγδ+CD3+T淋巴细胞含量及血液淋巴细胞的凋亡规律。结果:在出孵1个月内T、B细胞处于分化发育阶段的初期,除胸腺外,呈上升趋势,但T细胞亚群的变化无规律,B细胞在法氏囊中和T细胞在脾脏中3~9日龄发育最快;血液淋巴细胞凋亡趋于下降,尤其是在9日龄以后。此间不断有成熟的淋巴细胞产生,不断进入血液和脾脏,使其数量有上升趋势。但是,血液中第7日龄时Bu-1a+B淋巴细胞和CD3+、CD4+、CD8+T淋巴细胞数量均最低,说明体液免疫和细胞免疫水平第7日龄时都最低,并在17日龄时进入水平较高的又一个低谷。TCRγδ+CD3+T细胞则变化幅度不大。更多还原

【Abstract】 In order to verify the development of T & B lymphocytes and subsets of the T cell in chickens within 1 month, SPF chickens were killed in 3,5,7,9,11,14,17,22,27,32 days respectively. Lymphocytes were separated from blood, spleen, thymus and bursa of chickens. Then the content of Bu-1a~+B cell, CD3~+, CD4~+, CD8~+and TCRγ~+CD3~+ T cells and apoptosis of blood lymphocyte were checked by using flow cytometry with double-color-staining method in different days, respectively. The results showed: T & B lymphocytes were in the early stage of differentiating, maturing and were tending a increasing way except thymus within 1 month; but found no regularity in changes of T cell subsets. The speed of developing and maturing of B lymphocyte in bursa and T lymphocyte in spleen is the most rapidly in 3-9 days. Apoptosis of blood lymphocyte were decreasing especially 9th day later. They demonstrated T & B lymphocytes were in differentiating and developing in thymus and bursa continually, and then entering into spleen and blood, made matured lymphocyte content increasing within 1 month. But Bu-1a~+B cell and CD3~+T,CD4~+T,CD8~+T were in the lowest level in blood at 7th day, expressed lowest humeral and cellular immunological level at 7th day, then went to another lower at 17th day. TCRγ~+CD3~+T lymphocyte changing in a stable pattern.更多还原