【摘要】 在表达鸡传染性喉气管炎病毒(ILTV)糖蛋白gB基因和新城疫病毒(NDV)F基因的重组鸡痘病毒(rF-PV-gB-F)安全性检验合格后,以5.0×101~5.0×104PFU不同含量按0.1mL/鸡的剂量免疫100只30日龄SPF鸡,30d后分组分别用ILTVWG株和NDVF48E9株强毒进行攻击。免疫鸡抗鸡痘病毒抗体都转为阳性,痘反应和接种剂量有关,重组疫苗的最小反应剂量为50PFU。重组疫苗可以诱发对新城疫和传染性喉气管炎的保护,0.1mL/鸡的接种量在500~5000PFU浓度范围内的免疫效果最好,对于ILTV攻击的发病保护率在70%以上,对NDV强毒攻击的抗死亡保护率可以达到80%,这为进一步考察疫苗的免疫效力试验以及进行田间试验奠定了基础。更多还原

【Abstract】 The safety of recombinant fowlpox virus (rFPV-gB-F) expressing glycoprotein B (gB) gene of infectious larngotracheitis virus (ILTV) and F gene of Newcastle disease Virus(NDV) were evaluated. 100 30-day-old SPF chickens were divided into five groups, and inoculated with 50~510~4 PFU/0.1 mL rFPV-gB-F, respectively. 30 days after inoculation, chickens were divided and challenged with ILTV of Wanggang strain and NDV of F48E9 strain, respectively. Antibody titer to FPV was all positive in a dose dependent manner. And in the all range of all the inoculation, it can protect chickens against the challenge of the virulent ILTV and NDV, 7/10 chickens can be protected from clinical symptom in all the inoculation group, 8/10 of chickens inoculated survived from lethal challenge of NDV F48E9 in 500~5000PFU inoculation group, all these results lay the foundation for the further evaluation the efficiency of rFPV-gB-F.更多还原