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禽流感病毒M2基因在原核系统中表达及其抗原性分析

Expression of Avian Influenza Virus M2 Gene in E.coli and Analysis of Its Antigenicity

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【作者】 李永清杨敬韦莉杨汉春

【Author】 LI Yong-qing~(1,2), YANG Jing~3, WEI Li~2, YANG Han-chun~(1*) (1. College of Veterinary Medicine, China Agricultural University,Beijing 100094,China; 2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089,China;3. Laboratory Animal Center, Academy of Military Medicine Sciences, Beijing 100071,China)

【机构】 中国农业大学动物医学院军事医学科学院动物中心北京市农林科学院畜牧兽医研究所中国农业大学动物医学院 北京100094北京市农林科学院畜牧兽医研究所北京100089北京100071北京100094

【Abstract】 Avian influenza virus (AIV) is a highly variable pathogen. The M2 gene of AIV is a transmembrane protein. It has highly conservative antigenic epitopes and is a potential antigen for cross-protection. In this study, the recombinants containing the full-length M2 and the transmembrane segment deleted M2 were constructed respectively. When measuring the expression of the two constructs, we found that the full-length M2 failed to express in the prokaryotic expression system, whereas transmembrane segment deleted M2 was highly expressed as fusion protein in a soluble form. The fusion protein reacted with SPF chicken serum specific for AIV A/goose / Guangdong /1996(H5N1). The protein was purified by GST purification system and the purified protein was used to prepare anti-M2 serum in mice. Immunofluorescence test demonstrated the binding of the antiserum with AIV (H5N1) infected MDCK cells. The results suggest that the recombinant transmembrane segment deleted M2 could provide good antigenicity.

【关键词】 禽流感病毒M2基因抗原性
【Key words】 avian influenza virusM2 geneantigenicity
【基金】 北京市自然科学基金(5002006)
  • 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,2005年07期
  • 【分类号】S852.65
  • 【被引频次】10
  • 【下载频次】250
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