【摘要】 采用PCRRFLP技术,对200只寿光鸡的胰岛素样生长因子Ⅰ(IGFⅠ)基因的5′端调控区进行遗传多态性研究,该调控区的扩增产物经PstⅠ酶切后出现AA、AB和BB3种基因型,其基因型频率分别是0.335、0.485和0.180。经χ2检验,寿光鸡在IGFⅠ位点上处于HardyWeinberg平衡状态(P>0.05)。分析基因型与10周龄体重和部分屠体性状时发现:基因型对半净膛重和胸肌重有显著影响(P<0.05)。公鸡中在半净膛重和胸肌重上基因型BB与AB、AA之间存在显著差异(P<0.05),而母鸡群体中却在肝脏重和半净膛重上基因型BB同AB、AA之间存在显著差异(P<0.05)。在研究指标中寿光鸡的BB基因型效应均大于AB和AA基因型。更多还原

【Abstract】 Genetic variation of the 5′ region of the IGF-Ⅰgene in 200 Shouguang chickens were detected by PCR-RELP. Three genotypes AA,AB and BB were identified after the PCR products were cut by PstⅠrestriction enzyme and their frequencies were 0.335,0.485 and 0.180, respectively. They were in Hardy-Weinberg equilibrium at the gene locus by χ~2 test(P>0.05). The associations were observed between genotypes and carcass traits including semi-eviscerated weight and breast muscle weight (P<0.05). The results of the least squares means showed that a significant different effect between genotype BB and AB or AA was found in semi-eviscerated weight of Shouguang chickens (P<0.05), while the significant difference was found only in breast muscle weight of the male chickens and only in liver weight of female chickens (P<0.05). In addition, genotype BB tended to express a higher body weight in 10 weeks, semi-eviscerated weight and breast muscle weight than the other genotypes in Shouguang chickens. These results may be applied to marker assisted selection.更多还原