【摘要】 参照已发表的捻转血矛线虫H11蛋白基因序列设计引物,以H.contortusZJ株的总RNA为模板,进行RT2PCR扩增,成功扩增出H11基因。将PCR产物与pUCm2T载体连接后,转化DH5α感受态细胞,筛选阳性克隆并测序。序列分析表明,其核苷酸序列与国外报道的H11基因的同源性为99.5%。编码氨基酸序列具有氨基肽酶的保守序列,推测可使虫体不能获得所必需的氨基酸而导致死亡。更多还原

【Abstract】 In order to clone the H11 gene, the total RNA of Haemonchus contortus ZJ strain was extracted and used as template of RT-PCR. A pair of primers was designed according to the published data. After RT-PCR, the cloned H11 was ligated to the vector pUCm-T and transformated DH5α competent cell. The positive clones were verified by sequencing. Sequence analysis showed that the gene obtained was 99.5% (identical) to the H11 gene in the database. According to the sequence analysis, the deduced protein of H11 may has the function similar to aminopeptidase which make parasites dead from lack of necessary amino acids.更多还原