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RAPD分析山西主要地方山羊品种的遗传多态性

Genetic Variation of Shanxi Native Goat Breeds by RAPD Analysis

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【作者】 杜美红李步高周忠孝

【Author】 DU Mei-hong~1, LI Bu-gao~2, ZHOU Zhong-xiao~2(1.College of Biological Sciences, China Agricultural University,Beijing 100094,China;2.College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China)

【机构】 中国农业大学生物学院山西农业大学动物科技学院山西农业大学动物科技学院 北京100094太谷030801太谷030801

【Abstract】 Random Amplified Polymorphic DNA (RAPD) analysis was used with 10 primers to assess genetic variability in Shanxi native goats (Yangcheng White,Licheng Daqing,Lüliang Black). The results showed as follows: 1.The DNA of 3 breeds provided a great number of molecular markers with high polymorphism. 2. According to their RAPD fingerprinting map, the genetic distance and average band similarity among 3 breeds were calculated, 0.182 7 between Yangcheng White Goat and Licheng Daqing Goat is the smallest. Cluster analysis also showed that Licheng Daqing Goat and Yangcheng White Goat from alike region had a smaller distance. 3. The genetic diversity and its partition within and between populations of the 3 populations were calculated by using Shannon index. The genetic diversity index of Licheng Daqing,Yangcheng White,Lüliang Black were 0.328 8,0.349 9 and 0.154 5 respectively, Genetic differentiation (Gst) was estimated to be about 68.49 among 3 populations. The results indicated that the genetic variation was mainly among breeds; the genetic character of Lüliang Black Goat was peculiar in native breeds indicating that protection and further selection should be strengthened.

【基金】 山西省青年基金(20001041)
  • 【文献出处】 畜牧兽医学报 ,Chinese Journal of Animal and Veterinary Sciences , 编辑部邮箱 ,2005年02期
  • 【分类号】S827.2
  • 【被引频次】6
  • 【下载频次】104
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