【摘要】 目的　探讨后发性白内障与人工晶状体袢角度之间的关系。方法　 9只科研兔随机分为 3组 ,每组 6眼。麻醉后 ,行透明晶状体囊外摘出术 ,并分别植入袢角度不同的PMMA人工晶状体 ,第 1组植入 0°袢人工晶状体 ,第 2组植入 5°袢人工晶状体 ,第 3组植入 10°袢人工晶状体。术后 6个月 ,裂隙灯下观察晶状体后囊混浊情况并分级 ,取出晶状体后囊 ,行光镜及透射电镜检查 ,并采用免疫组化法对晶状体后囊染色 ,然后采用医用多功能图像分析软件对晶状体后囊表面及赤道部的增殖细胞核抗原 (proliferatingcellnucle arantigen ,PCNA)进行定量检测 ,用SPSS统计软件分析结果。结果　裂隙灯下可见术后随着人工晶状体袢角度的增加 ,发生后发性白内障的例数及等级均呈下降趋势。光镜及透射电镜扫描可见发生后发性白内障的晶状体后囊表面有一层或多层晶状体上皮细胞生长 ,未发生后发性白内障的晶状体后囊表面无晶状体上皮细胞生长 ,赤道部均可见多层晶状体上皮细胞生长。PCNA定量结果 :植入 0°袢人工晶状体组与植入 5°袢人工晶状体组之间后囊表面及赤道部PCNA阳性率 (分别为 10 .5 0 0±2 2 5 8,9.16 7± 2 .2 2 9)无明显差异 (P >0 .0 5 ) ,而植入 10°袢人工晶状体组 (4.5 0 0± 1.871)与植入 0°袢人工晶状体组及植入更多还原

【Abstract】 Objective To investigate the correlation between the different haptic angular magnitude of intraocular lens(IOL)and the incidence of posterior capsular opacification(PCO).Methods Nine rabbits were divided into 3 groups randomly,each group having 6 eyes.The rabbits were anesthesized and ECCE were performed on all eyes,then IOL with different haptic angular magnitude were implanted into each eye(0°the first group,5°the second group and 10°the third group).Six months later,PCO were graded under the slit lamp.All the posterior capsules extracted were examined by light microscope and transmission electron microscope.At the same time,the posterior capsules were dyed by immunohistochemistry,then the rate of the positive proliferating cell nuclear antigen (PCNA) both on posterior capsules and on equatorial posterior capsules were estimated.Results Under the slit lamp,with the increasing of haptic angular magnitude of IOL implanted,it showed a decline in the number and the grades of PCO.Under the light microscope and transmission electron microscope,the surfaces of posterior capsules with PCO were covered by a layer or a few layers of lens epithelium and no cell was found on posterior capsule surfaces without PCO.There were a few layers of lens epithelium on the surfaces of the equator posterior capsules.The rate of positive PCNA:the value of 10°haptic angular magnitude group differed from those of 0°haptic anglulation group and 5°haptic angular magnitude group significantly both on posterior capsules and on equatorial posterior capsules(P<0.05)(positive rates in each group were 10.500±2 258,9.167±2.229 and 4.500±1.871,respectively).The values of 0°haptic angular magnitude group and 5°haptic angular magnitude group had no difference both on posterior capsules and on equatorial posterior capsules(P>0.05).Conclusion PCNA is a convincible indicator to appraise the proliferation of cell.With the increasing of haptic angular magnitude of IOL implanted,the incidence of PCO dropped significantly.更多还原