【摘要】 目的:观察肝缺血再灌注损伤(HIRI)过程中脑组织中Fos和一氧化氮合酶(NOS)的变化,探讨两者在脑组织不同部位的改变及可能的作用。方法:建立肝缺血再灌注(I/R)损伤动物模型,分为对照组、缺血30min组(I组)、缺血30min再灌注组(I/R组),I/R组又分为再灌注后1h组(I/R1h)、再灌注后2h组(I/R2h)、再灌注后4h组(I/R4h),应用免疫组化法分别测定各组大鼠不同部位脑组织Fos和NOS的变化。结果:肝缺血再灌注损伤的不同时段可出现脑组织不同部位Fos和NOS改变,尤其在再灌注后期表现明显。结论:HIRI状态下,Fos和NOS在脑内参与了损伤过程。下丘脑中两者的变化在中枢调节作用中可能起着中介作用。更多还原

【Abstract】 Objective: To observe the changes of nitric oxide synthase (NOS) and fos protein in injuried brain following liver ischemia-reperfusion injury and evaluate the contribution of NOS and c-fos protein to brain tissue effect. Methods: A model of liver ischemia-reperfusion injury was established by imitation. The contents of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions were examined individually by immunohistochemical technique following sham operation (group 1), ischemia 30 min without reperfusion(group 2), reperfusion following schemia 30 min (group 3). 1 hour reperfusion following ischemia 30 min (group 4). 2 hour reperfusion following ischemia 30 min (group 5) and 4 hour reperfusion following ischemia 30 min (group 6). Results: Compared with group1, group2 and group3, the contents of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions were little statistic significance, (P>0.05). There was obviously statistic significance between group4 and former groups, (P<0.05). The increase exists remarkably about the contents of NOS and c-fos protein in hypothalamus compared with former groups in themselves (P<0.01).There also was higher level in group5 than the former groups (P<0.05), while no changes happen between group5 and group6(P>0.05). Conclusion: The data of NOS and c-fos protein in brain tissue including cortex hippocampus and hypothalamus regions vary depending on different time of liver ischemia-reperfusion specially, after reperfusion. NOS and c-fos protein are involved in brain tissue injury following reperfusion of ischemia liver.更多还原