【摘要】 目的:建立一条从妊娠早期妇女血清中分离纯化早孕因子的技术路线,获得高纯度的早孕因子。方法:采用依次经过DEAE52阴离子交换层析、SPSepharoseF.F阳离子交换层析、ConASepharose4B亲和层析和HeparinSepharoseCl6B亲和层析分离纯化的方案,最终得到具有早孕因子活性的HeparinⅡ成分,早孕因子活性采用活性玫瑰花环抑制实验检测。采用SDS聚丙烯酰胺凝胶电泳鉴定纯化物。结果:SDS聚丙烯酰胺凝胶电泳结果显示有一条电泳区带,相对分子量为10.91kDa。结论:这条分离纯化早孕因子的技术路线是可行的,得到的相对分子量为10.91kDa的蛋白质为电泳纯的早孕因子。更多还原

【Abstract】 Objective: Set up a method of isolation and purification of early pregnancy factor(EPF) from human pregnancy sera, and get pure EPF. Methods: The purification scheme involved sequential DEAE 52 chromatography, SP Sepharose F.F chromatography, ConA Sepharose 4B chromatography and Heparin Sepharose Cl-6B chromatography. The biological activity was detected by rosette inhibition test. It was (Identified) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Result: One band was observed on SDS-PAGE profile, and the molecular weight was 10.91 kDa. Conclusion: This method is advisable. The purified protein has a molecular mass of 10.91 kDa as analyzed by SDS-PAGE, which is similar to the report of Cavanagh AC.更多还原