【摘要】 探讨MDV(Marek’sDiseaseVirus)抗独特型抗体(Ab2)模拟抗原诱导抗MDV感染的能力。将MDV抗独特型抗体分别与福氏完全佐剂和福氏不完全佐剂按1∶1比例乳化制备成抗体苗,对1日龄,10日龄SPF鸡进行两次免疫,同时设火鸡疱疹病毒疫苗(HVT)和非免疫空白对照。30日龄后用马立克氏病毒(MDV)标准Ⅰ型强毒———京1毒行腹腔攻击,在第30天,第60天,第120天采血,分别检测免疫系统—淋巴器官重量、循环血淋巴细胞数量、病理组织学变化、孵化率及免疫抑制性反应。MDV抗独特型抗体苗免疫攻毒后淋巴组织器官增重、器官未见淋巴细胞浸润,120d保护率为83.45%,未见大肠杆菌继发感染发生。HVT苗免疫攻毒后淋巴组织器官,淋巴细胞的数量下降、出现病理损伤,120d保护率为73.80%。MDV感染法氏囊、脾脏的重量及循环血中淋巴细胞的数量减少,大肠杆菌的敏感性增加。MDV抗独特型抗体能诱导机体产生抗MDV感染保护。更多还原

【Abstract】 The study was conducted on immunity protective efficacy of anti-idiotypic antibodies (Ab2) against chicken marek’s disease virus (MDV). The chickens (SPF) were immunized with Ab2 of freund’s complete adjuvant or incomplete adjuvant two times at the age of 1 day and 10 days respectively, and then were challenged with virulent MDV at the postinoculation 30 days. Immunity protective efficacy was observed by immunology system state with lymphoid organs weight, lymphocyte on circular blood, lymphoproliferation, hatch rate at the different period and anti-immunosuppressive action agaist E.coliform infection. The results showed that Ab2 did not induce histopathology changes on the lymphoid tissues, hatch had low death rate, liver and spleen had not seen lymphoid cell invade and immune protective rate was 83.45% . Opposition induce histopathology changes of HVT on the lymphoid tissues were more Ab2, hatch had low death rate, liver and spleen had seen lymphoid cell invade and immune protective rate was 73.80%, and induce histopathology and anti-immunosuppressive action with infection E.coliform on MD contrast were more serious than any one on day 120 post-MDV challenge. Vaccination with anti-idiotypic antibodies induce strong protection against marek’s disease viruses infection .更多还原