【摘要】 运用随机扩增多态性DNA(RAPD)和inter简单重复序列(ISSR)等分子标记技术,分别对5组花椰菜自交不亲和系和对应的自交亲和系的基因组进行指纹差异分析.采用10个10mer随机引物和5个ISSR引物进行PCR扩增,结果表明,扩增片段分子量在0.3～5kb之间,指纹图谱的稳定性和重复性较好.经过3次以上重复发现,ISSR4引物扩增图谱中,自交亲和系比不亲和系多扩增出3800bp片段;ISSR6引物扩增图谱中,除第3组外,不亲和系比亲和系多扩增出1100bp片段.结果表明,花椰菜自交不亲和系与自交系基因组DNA之间存在差异,扩增出的差异片段与花椰菜自交不亲和性相关,并可作为自交不亲和系和自交亲和系育种筛选的分子标记.初步探讨了花椰菜自交不亲和性的遗传机制及RAPD和ISSR技术在自交不亲和系选育过程中的应用前景.更多还原

【Abstract】 Genetic differences between self-incompatible and correspondent self-compatible among five lines of cauliflowers were analyzed by using random amplified polymorphism DNA (RAPD) and inter-simple sequence repeats (ISSR).Ten selective primers were used in RAPD analysis and five selective primers were used in ISSR analysis.The results show that the molecular weight of amplified fragments were between 0.3 kb and 5 kb,and the genetic fingerprint could be reproductive for more than 3 times.It was found that the self-compatible lines amplified a 3 800 bp fragment with ISSR4 primer but self-incompatible lines can not;the self-incompatible lines amplified a 1 100 bp fragment with ISSR6 primer but self-incompatible lines can not except one group.The different bands were related to cauliflower self-incompatibility.The genetic mechanism of cauliflower self-incompatibility and the application of RAPD and ISSR in breeding of self-incompatibility cauliflower were preliminary discussed.更多还原