【摘要】 以癌细胞诱导分化物维甲酸为平行对照,应用中药有效成份熊去氧胆酸处理人成骨肉瘤MG63细胞,观察比较熊去氧胆酸及其与维甲酸的组合对MG63细胞形态、细胞周期及分化的影响.实验结果显示,经熊去氧胆酸、维甲酸及其组合联合处理之后,MG63细胞体积增大,核质比例减少,形态趋于规则.细胞周期发生G0/G1期阻滞,G0/G1期细胞比例显著增加,而S期和G2/M期细胞比例则明显下降.细胞终末分化标志物I型胶原表达明显增强,骨结节形成数目增多,且结节结构更为典型.其中,熊去氧胆酸和维甲酸联合组对MG63细胞周期的阻滞及细胞终末分化指标I型胶原的表达、骨结节形成的诱导均强于单独处理组.结果表明,熊去氧胆酸具有与维甲酸相近似的诱导人成骨肉瘤MG63细胞分化的作用,并且两者组合具有协同诱导成骨肉瘤细胞分化的效果.更多还原

【Abstract】 In this study,the human osteosarcoma MG-63 cells were treated with ursodeoxycholic acid,retinoic acid.The combination of the two drugs was used to observe and to compare their effects on the morphology,cell cycle and differentiation of human osteosarcoma MG-63 cells.The results showed that after the treatment of ursodeoxycholic acid,retinoic acid and their combination,MG-63 cells became bigger.The proportion of nucleus to cytoplasm decreased and the cell morphology was more regular.The cell cycle of MG-63 cells was arrested at G0/G1 stage and the cells in this stage increased markedly,while the cells in S and G2/M stages decreased greatly.The expression of the terminal differentiation maker,collagen I,was upregulated.The number of bone nodule,which is another important terminal differentiation marker,increased and the morphology and structure of the bone nodules were typical.Compared with ursodeoxycholic acid and retinoic acid,their combination had better effects on the cell cycle arrest,the expression of collage I and the induction of bone nodules formation.From the above,a conclusion can be drawn that,ursodeoxycholic acid has similar effect on inducing human osteosarcoma MG-63 cells to differentiation.And their combination has better effect on the differention induction of MG-63 cells更多还原