【摘要】 实时荧光定量PCR技术是一种利用不同的荧光检测来给核酸定量的技术。目前 ,该技术已经应用于临床微生物、基因表达、肿瘤免疫、微小残留病变的检测 ,DNA拷贝数的测量、基因组变异和多态性等许多方面。现主要介绍该技术的一些基本原理及其应用。更多还原

【Abstract】 Real-time fluorescent quantitative PCR(FQ-PCR) is a kind of techniques that can quantitate the nucleic acid based on different fluor escence.Currently FQ-PCR has been widely used in many fields such as clinical m icrobiology,gene expression,tumor immunology,detection of minimal residual lesio n,measurement of DNA copy number,genome mutation,polymorphism and so on.In this paper the basic principles and the applications of this technology are introduce d.更多还原