节点文献
胞内钙离子浓度的定量检测软件的设计与实现
Software Design and Realization for the Fluorescent Microscope System to Measure [Ca2+]i in Living Cell
【摘要】 钙离子光解释放与Fura荧光探针技术目前在生理学研究领域有着广泛的应用,目前国外已有能够检测活体细胞内钙离子浓度的装置和软件,但是全套实验配置价格昂贵,非常规实验室可接受,而国内这方面的研究开发又尚属空白。介绍了在自行研制的单色光源的基础上,基于VC++6.0开发的荧光测钙软件。根据生理实验的要求,设计实现了荧光测钙实验的各个功能模块,初步的实验结果表明:该软件具有功能清晰、人机界面友好,使用方便等特点,如若完善,有望将来取代国外同类产品,从而降低国内生理学研究的成本。
【Abstract】 A new software for the fluorescence microscope based on vc ++6.0 to measure [Ca2+]i in living cell is discussed. Several modules of function has been designed according to the requirement of experiment for measuring [Ca2+]i .The technol- ogy of object oriented in vc++ and the interface design which has been validated in experiments are circumstantiated in the article.
【关键词】 荧光测钙；
面向对象；
VC++；
ActiveX；
【Key words】 Measurement [Ca2+]i by microfluoremetric technique； object oriented； VC++； ActiveX This work was supported by grants of the General Program (No.30327001, 60071030) and the Key Program (No. 30130230) from the National Natural Science Foundation of China.；
【Key words】 Measurement [Ca2+]i by microfluoremetric technique； object oriented； VC++； ActiveX This work was supported by grants of the General Program (No.30327001, 60071030) and the Key Program (No. 30130230) from the National Natural Science Foundation of China.；
【基金】 国家自然科学基金资助项目(NO.30327001)
- 【文献出处】 现代科学仪器 ,Modern Scientific Instruments , 编辑部邮箱 ,2005年03期
- 【分类号】TP311.52
- 【下载频次】85