【摘要】 目的:研究食品中沙门菌的整合子携带情况及整合子与耐药性之间的关系。方法:PCR检测细菌总DNA中1、2、3类整合酶基因,确定细菌携带整合子的情况。整合子阳性菌档进一步检测整合子的可变区,分析插入基因盒的序列。结果:69株大肠菌中25株携带1类整合子,集中分布在对4种以上抗生素耐药的菌株中。插入基因盒主要是dfr(甲氧苄氨嘧啶)和aadA(氨基糖甙类)基因盒,各种基因盒组合中最常见的是dfrl-aadA1。结论:细菌的多重耐药性与整合子携带高度一致,但是单个菌株的耐药谱与整合子的耐药基因盒缺乏对应关系。更多还原

【Abstract】 Objective:To investigate the relationship between antibiotic resistance of Salmonella spp strains isolated from food and the integrons they harbored.Methods:PCR amplifying intl gene and the variable region between the conserved segments(CS-PCR) were performed for class l,class 2,and class 3 integron.And the integron content of every isolate was characterized by RELP analysis and sequencing.Results:25 of 69 isolates were identified as being positive for integrase 1 gene in the total DNA. Most of the integron-positive strains were resistant to more than 4 antibiotics.Most of the gene cassettes integrated in the integrons conferred resistant against trimethoprim and streptomycin-spectinomycin,and the integrons with the dfrl-aadA1 gene cassettes combination were predominant.Strains with different antibiotic resistance profile of plasmid profile might have the same integron.Conclusions:There was a good correlation between integron-carrying and the antibiotic resistance of Salmonella spp.strains isolated from food.更多还原