【摘要】 目的　建立高效液相色谱法分析血浆中盐酸二甲双胍浓度的方法。方法　分析柱为KromasilC18 ( 250mm×4. 6mm,5μm),流动相为乙腈:磷酸缓冲液(内含少量十二烷基磺酸钠 ) 40∶60,UV检测波长为 233nm,采用高氯酸沉淀蛋白的方法,以吗啉双胍为内标。结果　测定二甲双胍的线性范围为 0. 050~4. 00μg·mL-1,日内精密度<8. 18%,日间精密度 <8. 74%,方法回收率均>90%。结论　本方法精密,准确,简便,适用于二甲双胍药动学的研究。更多还原

【Abstract】 OBJECTIVE A modified procedure has been established for the determination of metformin in human plasma.METHOD A Kramsil C 18 colum was used with a mobile phase of acetonitrile-phosphate buffer（60∶40）,detected at 233nm.The samples were deproteinated with HClO₄,MLSG was used as the internal standard.RESULTS The calibration curve was linear over the range of 0.050⁴.00μg·mL -1.The inter-day RSD was less than 8.18% and the intra-day RSD was less than 8.74%.The recovery was 92.05%¹02.01%.CONCLUSION This method is simple with high sensitivity and good precision and is suitable for pharmacokinetic studies of metformin in humans.更多还原