【摘要】 目的:观察兔耳慢性缺血性溃疡愈合过程中增殖细胞核抗原、半胱氨酸天冬氨酸蛋白酶3和c-myc的表达变化,了解增殖与凋亡活动对慢性缺血性创面愈合的作用。方法:实验于2003-10/2004-10在昆明医学院第二临床学院中心实验室完成。选用健康日本纯种大耳白兔23只,对照组2只,为无缺血兔耳正常皮肤;实验组21只,建立慢性缺血性兔耳溃疡模型。采用兔耳慢性缺血性溃疡模型,设立伤后1,4,7,11,15,22和26d组,用SP免疫组织化学方法研究创面组织中增殖细胞核抗原、半胱氨酸天冬氨酸蛋白酶3和c-myc蛋白的表达规律和分布特征。结果:实验兔23只均进入结果分析。①创面愈合过程中增殖细胞核抗原、半胱氨酸天冬氨酸蛋白酶3和c-myc蛋白的表达增高,并具有一定的时相性。②正常兔耳皮肤组织中有少量的增殖细胞核抗原、半胱氨酸天冬氨酸蛋白酶3和c-myc蛋白的表达。③伤后15d,增殖细胞核抗原、半胱氨酸天冬氨酸蛋白酶3和c-myc蛋白的表达最高,随后逐渐下降,主要分布在成纤维细胞的胞浆和胞核。结论:创面慢性缺血性溃疡愈合过程中增殖细胞核抗原、半胱氨酸天冬氨酸蛋白酶3和c-myc蛋白的表达升高,其表达具有时相特征性和特定的表达部位,三者表达的时相提示半胱氨酸天冬氨酸蛋白酶3和c-myc参与调控慢性缺血性溃疡愈合过程,细胞增殖活动与细胞凋亡的规律基本一致。适量调控半胱氨酸天冬氨酸蛋白酶3和c-myc的表达水平,可能促进创面愈合。更多还原

【Abstract】 AIM: To observe the changes in the expressions of proliferating cell nu-clear antigen(PCNA), caspase-3 and c-myc so as to study the effect of pro-liferation and apoptosis on the healing of chronic ischemic wound.METHODS: The experiment was conducted in the Central Laboratory ofthe Second Clinical College of Kunming Medical College from October2003 to October 2004. Twenty-three healthy Japanese flap-eared whiterabbits were selected and divided into two groups: two rabbits in controlgroup with non-ischemic ear skin and 21 in experimental group with chron-ic ischemic ulcer in ear. After establishment of chronic ischemic ulcermodel of ear in rabbits, the experimental group were subdivided into 1-, 4-,7-, 11-, 15-, 22-, and 26-day post-injury groups. SP immunohistochemicalmethod was used to study the expression regularity and distributing char-acteristics of PCNA, caspase-3 and c-myc protein.RESULTS: All the 23 rabbits were involved in the result analysis. ①Dur-ing the wound healing, expressions of PCNA, caspase-3 and c-myc proteinwere increased in a time-phase manner. ②There were few expressions ofPCNA, caspase-3 and c-myc protein in normal ear skin of rabbits. ③Fif-teen days after injury, expressions of PCNA, caspase-3 and c-myc proteinreached the peak, then decreased gradually, and mainly distributed in cy-toplasm and nuclei of fibroblasts.CONCLUSION: In the wound healing of chronic ischemic ulcer, expres-sions of PCNA, caspase-3 and c-myc protein are increased in a time-phasemanner and in definite sits. The expressions in a time-phase manner indi-cate that caspase-3 and c-myc are involved in the regulation of the healingof chronic ischemic ulcer, and the rule of cell proliferation is basically sim-ilar to that of cell apoptosis. Proper regulation of caspase-3 and c-myc ex-pression may facilitate wound healing.更多还原