【摘要】 目的:从酶屏障的角度观察乳酸脱氢酶抗体对大鼠血管源性脑水肿的干预作用。方法:实验于2005-06-10/07-10在南方医科大学附属南方医院神经内科实验室完成。①分组:60只清洁级Wistar大鼠随机分为正常组、脑水肿组、脑水肿甘露醇组与脑水肿乳酸脱氢酶抗体组,每组15只。每组取10只用于脑组织水分含量测定,其余5只用于脑毛细血管伊文思蓝渗出量测定。②模型制备及给药:用腹腔注射苯肾上腺素的方法制成血管源性脑水肿模型。正常组不注射苯肾上腺素及治疗药物,直接断头取脑;脑水肿组制备模型后不给予治疗药物,即刻断头取脑;脑水肿甘露醇组在制成模型后由股静脉注射甘露醇(20g/L,0.5g/kg),30min后断头取脑;脑水肿乳酸脱氢酶抗体组在制成模型后腹腔注射乳酸脱氢酶抗体(10mL/kg),30min后断头取脑。通过水分分析仪分别测定各组脑灰、白质水分含量百分比。用伊文思蓝测定血脑屏障的通透性。结果:60只大鼠均进入结果分析。①脑水肿甘露醇组和脑水肿乳酸脱氢酶抗体组脑灰、白质的水分含量百分比显著低于脑水肿组[灰质:(72.69±1.90)%,(72.78±1.34)%,(76.53±1.39)%,P<0.01;白质:(73.63±1.11)%,(69.58±2.73)%,(77.45±2.38)%,P<0.01]。脑水肿甘露醇组与脑水肿乳酸脱氢酶抗体组相比脑灰质水分百分比差异无显著性意义(P>0.05),白质水分含量差异有显著性意义(P<0.01)。②脑水肿甘露醇组和脑水肿乳酸脱氢酶抗体组伊文思蓝渗出量与脑水肿组相比有显著性下降[(0.0626±0.0033),(0.0441±0.0056),(0.0736±0.0032)A/g(P<0.01)]。与脑水肿甘露醇组相比,脑水肿乳酸脱氢酶抗体组伊文思蓝渗出量有显著下降(P<0.01)。结论:甘露醇可降低灰质、白质水肿,但降低血脑屏障通透性差。乳酸脱氢酶抗体对减少血管源性脑水肿大鼠脑灰、白质水分含量、降低血脑屏障通透性均有显著效果。血管源性脑水肿中血脑屏障的通透性改变与乳酸脱氢酶活性有关,乳酸脱氢酶抗体对血管源性脑水肿有治疗作用。更多还原

【Abstract】 AIM To observe the interventional effect of lactate dehydrogenase antibody on vasogenic brain edema in rats. METHODS The experiment was carried out in the neurological lab Nanfang Hospital affiliated to Southern Medical University from June to July 2005. ① Grouping Sixty Wistar rats of clean degree were randomly divided into normal group brain edema group n=15 Mannitol-treated group n=15 and lactate dehydrogenase antibody-treated group n=15. Ten rats in each group were used in the determination of water content in brain tissue the other 5 were used in the determination of Evans blue bleeding in cerebral blood capillary. ② Model establishment and administration Models of vasogenic brain edema were made by intraperitoneal injection of phenylephrine. In the normal group the rats were not injected with phenylephrine and received no drug therapy the heads were cut to remove brain directly. In the brain edema group the rats did not receive any drug therapy after model establishment and the heads were cut to remove brain immediately. In the Mannitol-treated group the rats were treated with femoral venous injection of Mannitol after model establishment and the heads were cut to remove brain after 30 minutes. In the lactate dehydrogenase antibody-treated group the rats were given intraperitoneal injection oflactate dehydrogenase antibody 10 mL/kg and the heads were cut to remove brain after 30 minutes. The percentages of water content in brain gray matter and white matter were determined with water analytic apparatus. The Evans blue EB was used to test the permeability of blood brain barrier BBB. RESULTS All the 60 rats were involved in the analysis of results. ① The percentages of water content in brain gray matter and white matter were significantly lower in the Mannitol-treated group and lactate dehydrogenase antibody-treated group gray matter 72.69±1.90% 72.78±1.34% 76.53±1.39% P < 0.01 white matter 73.63±1.11% 69.58±2.73% 77.45±2.38% P < 0.01. The percentage of water content in brain gray matter had no significant difference between the Mannitol-treated group and lactate dehydrogenase antibody-treated group P > 0.05 but that in white matter had significant difference P < 0.05. ② The permeabilities of EB bleeding in the Mannitol-treated group and lactate dehydrogenase antibody-treated group were significantly decreased as compared with that in the brain edema model group 0.062 6±0.003 3 0.044 1±0.005 6 0.073 6±0.003 2 A/g P < 0.01 and it was significantly decreased in the lactate dehydrogenase antibody-treated group as compared with that Mannitol-treated group P < 0.01. CONCLUSION Mannitol can reduce the edema in gray matter and white matter but have bad effect in decreasing the BBB permeability. Lactate dehydrogenase antibody has significant effect in reducing the water content in the gray matter and white matter and decreasing BBB permeability of rats with vasogenic brain edema. The BBB permeability changes of vasogenic brain edema is associated with the activity of lactate dehydrogenase lactate dehydrogenase antibody has a therapeutic effect on vasogenic brain edema.更多还原