【摘要】 目的:探讨五肽胃泌素对大鼠肺动脉压的影响及其所产生的中枢性作用。方法:实验于2003-10/2004-06在郧阳医学院机能实验中心完成。实验选用SD雄性大鼠56只,将大鼠随机分为6组。①生理盐水组(n=6):于侧脑室内注射生理盐水40μL连续观察30min。②侧脑室注射五肽胃泌素组(n=8):于大鼠侧脑室内注射五肽胃泌素0.2g/L。③生理盐水+五肽胃泌素组(n=6):于大鼠侧脑室预先注射生理盐水40μL,5min后从原导管注射五肽胃泌素0.2g/L,连续观察30min。④酚妥拉明+五肽胃泌素组(n=12):于大鼠侧脑室注射酚妥拉明((受体阻断剂)0.25g/L,连续观察30min肺动脉压,5min后从原导管注射五肽胃泌素0.2g/L。⑤普萘洛尔(β受体阻断剂)+五肽胃泌素组(n=12):于大鼠侧脑室预先注射普萘洛尔0.1g/L,5min后从原导管注射五肽胃泌素0.2g/L,连续观察30min。⑥阿托品(M受体阻断剂)+五肽胃泌素组(n=12):于大鼠侧脑室预先注射阿托品0.1g/L,5min后从原导管注射五肽胃泌素0.2g/L,连续观察30min。以肺动脉压为指标,在氨基甲酸乙酯麻醉、三碘季铵酚制动、呼吸机控制呼吸的SD大鼠上,观察给药前,给药后1,5,10,15,20,25,30min大鼠肺动脉压的变化。结果:56只大鼠均进入结果分析。①侧脑室注射五肽胃泌素组给药后5,10,15min大鼠肺动脉压明显高于给药前犤(20.6±1.0),(21.2±1.2),(20.4±1.0),(18.8±1.0),(18.7±0.8),(18.9±1.0)mmHg,P<0.01犦。②酚妥拉明+五肽胃泌素组大鼠在给药5,10,15min后肺动脉压明显低于生理盐水+五肽胃泌素组犤(18.6±1.1),(18.8±1.0),(18.6±1.1)mmHg;(20.1±1.0),(20.9±0.9),(21.1±1.0)mmHg,P<0.01犦。③普萘洛尔+五肽胃泌素组、阿托品+五肽胃泌素组大鼠在给药5,10,15min后肺动脉压与生理盐水+五肽胃泌素组比较,无明显差异犤(19.6±1.0),(20.4±1.1),(21.0±1.2)mmHg;(19.5±1.1),(20.6±1.2),(21.4±1.0)mmHg,P>0.05犦。结论:五肽胃泌素具有中枢性升高肺动脉压的作用,此作用可能部分由脑内β受体介导。更多还原

【Abstract】 AIM To study the effects of pentagastrin on the pulmonary arterial pressure in rats and discuss its central role.METHODS The experiment was carried out in the Functional Laboratory of Yunyang Medical College between October 2003 and June 2004.Fifty-six male SD rats were randomly divided into 6 groups ①saline groupn=6 rats received intracerebral ventricular injection of 40 μL saline and were observed for continuously 30 minutes.②intracerebral ventricular injection of pentagastrin groupn=8 rats received introcerebral ventricular injection of 20 g/L pentagastrin.③saline+pentagastrin groupn=6 rats received intracerebral ventricular injection of 40 μL salineand then they were injected with 0.2 g/L pentagastrin through previous catheter after 5 minutesand were observed for 30 continuous minutes.④phenolamine+pentagastrin groupn=12 rats received intracerebral ventricular injection of 0.25 g/L phenolaminealpha receptor blocking agentand the pulmonary arterial pressure was observed for 30 continuous minutesand then 0.2 g/L penta-gastrin was injected through previous catheter after 5 minutes.⑤propranololbeta receptor blocking agent+pentagastrin groupn=12 rats received intracerebral ventricular injection of 0.1 g/L propranololthen 0.2 g/L pentagastrin was injected through previous catheter after 5 minutesand they were observed for 30 continuous minutes.⑥atropineM receptor blocking agent+pentagastrin groupn=12 rats received intracerebral ventricular injection of 0.1 g/L atropineand then 0.2 g/L pentagastrin was injected through previous catheter after 5 minutesthey were observed for continuously 30 minutes.Taking pulmonary arterial pressure as the indexthe changes of pulmonary arterial pressure were observed before administration and 1510152025 and 30 minutes after administration in the SD ratswhich were anesthetized with ethyl carbamate and immobilized with syncurarineand whose breath was controlled with breathing machine.RESULTS All the 56 rats were involved in the analysis of results.①The pulmonary arterial pressure in the intracerebral ventricular injection of pen-tagastrin group was obviously higher at 510 and 15 minutes after adminis-tration than before administration20.6±1.021.2±1.220.4±1.018.8±1.018.7±0.818.9±1.0 mm HgP < 0.01.②The pulmonary arterial pressures at 510 and 15 minutes after adminis-tration in the phenolamine+pentagastrin group were remarkably lower than those in the saline+pentagastrin group18.6±1.118.8±1.018.6±1.1 mm Hg20.1±1.020.9±0.921.1±1.0 mm HgP < 0.01.③The pulmonary arterial pressures at 510 and 15 minutes after administration in the propranolol+pentagastrin group and atropine+pentagastrin group19.6±1.020.4±1.121.0±1.2 mm Hg19.5±1.120.6±1.221.4±1.0 mm Hg were not obviously different from those in the saline+pentagastrin groupP > 0.05.CONCLUSION Pentagastrin has an effect on the central increase of pulmonary arterial pressurewhich may be mediated by intracerebral beta receptor.更多还原