【摘要】 背景:糖尿病患者糖代谢紊乱与脂代谢异常互为因果,导致肝糖原减少和血浆多种因子改变,从而易致心、肾、脑等血管并发症的发生,寻找有效的糖尿病二级干预药物已成为当今研究的热门课题。目的:探讨雷竹笋汁对大鼠肝糖原、血浆葡萄糖及血脂的干预效应。设计:以实验动物为研究对象,随机对照观察研究。单位:咸宁学院药学院实验室。材料:Wistar雄性大鼠40只,体质量(230±20)g,由广州军区武汉总医院医学实验动物中心提供。方法:实验于2002-09/2003-06在咸宁学院药学院实验室完成采用四氧嘧啶(200mg/kg)腹腔注射制备糖尿病大鼠模型。分别用不同剂量的雷竹笋汁犤205.70mg/kg(雷竹笋-Ⅰ组)和436.40mg/kg(雷竹笋-Ⅱ组)犦灌胃28d,并以健康大鼠作正常对照。测定各组大鼠肝糖原含量和血浆多种因子水平。主要观察指标:各组大鼠肝糖原、血浆葡萄糖、三酰甘油、总胆固醇、非酯化脂肪酸(non-esterifiedfattyacid,NEFA)、低密度脂蛋白胆固醇(Lowdensitylipoproteincholesterol,LDL-c)、高密度脂蛋白胆固醇(highdensitylipoproteincholesterol,HDL-c)、丙二醛含量和超氧化物歧化酶(superoxidedismutase,SOD)活性。结果:雷竹笋-Ⅱ组大鼠血浆葡萄糖含量为(9.65±1.14)mmol/L、三酰甘油为(1.31±0.18)mmol/L、NEFA为(0.51±0.04)mmol/L、LDL-c为(0.79±0.19)mmol/L和丙二醛为(6.31±0.83)nmol/L显著低于模型组犤葡萄糖(16.14±1.59)mmol/L,三酰甘油(1.94±0.31)mmol/L,NEFA(0.59±0.05)mmol/L,LDL-c(1.34±0.28)mmol/L和丙二醛(10.95±2.90)nmol/L,(t=3.478～9.379,P<0.01)犦,而雷竹笋-Ⅱ组HDL-c为(1.09±0.13)mmol/L、SOD为(39.76±6.23)NU/ml和肝糖原为(6.70±0.62)mg/g显著高于模型组犤HDL-c(0.55±0.11)mmol/L,SOD(23.24±3.35)NU/ml和肝糖原(4.06±1.24)mg/g,(t=5.388～9.000,P<0.01)犦。而且,雷竹笋汁二个剂量组间LDL-c的降低作用和SOD及肝糖原的升高作用呈现剂量依赖关系(P<0.05～0.01)。结论:雷竹笋汁明显降低糖尿病大鼠血浆葡萄糖、三酰甘油、NEFA、LDL-c和MDA及升高HDL-c、SOD和肝糖原等因子水平,显示有效的抗糖尿病作用。更多还原

【Abstract】 BACKGROUND:Diabetic glycometabolism disorder and lipoid metabolism disorder are often reciprocal causation,which cause the reduction of hepatic glycogen and the changes of many plasma factors.Hence,the occurrence of vascular complications in heart,kidney and brain is common.It becomes a hot topic in latest researches to find an effective medicine for the secondary prevention of diabetes.OBJECTIVE:To study the interventional effects of leizhusun’s juice on hepatic glycogen, plasma glucose and blood fat.DESIGN:A randomized and controlled observatory study by employing experimental animals as subjects.SETTING:Laboratory of Pharmacy College of Xianning College.MATERIALS:Totally 40 male Wistar rats with a bodymass of (230± 20) g were obtained from the Experimental Animal Center of Wuhan General Hospital of Guangzhou Military Area.METHODS:The diabetic rat model was established by the injection of Alloxan(200 mg/kg) into abdominal cavity in the laboratory of Pharmacy College of Xianning University between September 2002 and June 2003.Leizhusun’s juice(LZS) of different doses(205.70 mg/kg and 436.40 mg/kg) was respectively perfused into the stomach for 28 days, and healthy rats were used as normal control. Levels of hepatic glycogen(HG) in liver and the factors in plasma were detected.MAIN OUTCOME MEASURES:HG,glucose(Glu),triacylglycerol (TG),non esterified fatty acid(NEFA),total cholesterol(TC), low density lipoprotein cholesterol(LDL c),high density lipoprotein cholesterol(HDL c),and malondialdehyde(MDA) contents, and superoxide dismutase(SOD) activities RESULTS:The level of Glu in LZS II group(436.40 mg/kg) was (9.65 ± 1.14) mmol/L,TG was(1.31± 0.18) mmol/L,NEFA was(0.51± 0.04) mmol/L,LDL c was(0.79± 0.19) mmol/L, and MDA was(6.31± 0.83) nmol/L,which were significantly lower than Glu of (16.14± 1.59) mmol/L,TG of(1.94± 0.31) mmol/L,NEFA of(0.59± 0.05) mmol/L,LDL c of(1.34± 0.28) mmol/L and MDA of(10.95± 2.90) nmol/L in experimental control(Model) group(t=3.478- 9.379, P< 0.01),while the HDL c was(1.09± 0.13) mmol/L,SOD was(39.76± 6.23) NU/mL and HG was(6.70± 0.62) mg/g in LZS II group,which were significantly higher than HDL c of(0.55± 0.11) mmol/L,SOD of (23.24± 3.35) NU/mL and HG of(4.06± 1.24) mg/g in Model group(t=5.388- 9.000,P< 0.01). Furthermore, the decreasing effect on LDL c and increasing effect on SOD in both LZS groups had dose dependence relationship(P< 0.05- 0.01).CONCLUSION:LZS significantly decreases plasma Glu,TG,NEFA, LDL c and MDA levels, but increases HDL c,SOD and HG levels, which displays obvious antidibetic effects.更多还原