【摘要】 背景:中医对类风湿关节炎rheumatoidarthritis,RA的认识和治疗积累()比较丰富的经验,尤其是经过近几年来的研究,取得了令人瞩目的进展,具有治疗方法多,疗效确切,毒副作用少的特点,因而从中医领域进一步探寻治疗RA更为有效的药物和方法,有着重要的科学意义和社会意义。而中药能否在RA发病中通过多途径、多靶点、多环节作用方式,抑制或阻止软骨和骨的破坏并促进他们的新陈代谢及其损伤的修复的研究更是少有报道。目的:探讨中药通痹灵抗软骨破坏的作用机制。设计:以实验动物为研究对象的完全随机设计,对照实验研究。单位:一所大学金匮教研室。材料:实验于2002-08/2003-12在广州中医药大学第一附属医院综合实验室完成。Wistar清洁级大鼠,雌性,体质量(110±20)g,35只。由解放军第一军医大学动物实验室提供,动物合格证号:2002A041。将Wistar大鼠适应性喂养3d后,随机分为正常组、造模组、通痹灵总碱组。通痹灵总碱由广州中医药大学热带病研究所植物化学分析室提取。方法:以胶原诱导型关节炎大鼠为动物模型,分组灌胃治疗后,做大鼠趾关节病理切片,常规苏木精-伊红染色,观察软骨病变;培养软骨细胞,以rrIL-1β体外刺激软骨细胞导致软骨细胞基质降解作为药理模型,加用不同浓度的通痹灵总碱,并以地塞米松作为对照组,?更多还原

【Abstract】 BACKGROUND:Chinese medicine is rich in recognition and treatment for rheumatoid arthritis(RA) and has achieved attractable progression in researches of recent several years,characterized by variable therapeutic methods,definite therapeutic effects and few toxic side effects.Therefore,it is important scientifically and socially to further search for the effective drugs and methods for the treatment of RA in the field of Chinese medicine.There are few reports on whether Chinese herbs inhibit or block the destruction of cartilage and bone and promote their metabolism and repair for damage or not by variable approaches,targets and links in RA occurrence.OBJECTIVE:To probe into the mechanism of Chinese herbal tongbiling on cartilage destruction.DESIGN:Complete random design and control experimental study based on the experimental animals.SETTING:Golden Chamber teaching and research room of guangzhou university of traditional Chinese medicine.MATERIALS:The experiment was performed in Comprehensive Experimental Room of First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from August 2002 to December 2003,in which,35 female Wistar rats were employed,clean grade, weighted(110± 20) g,provided by Animal Experimental Room of First Military Medical University of Chinese PLA,with qualified animal No.2002A041.After 3 days adaptive breeding,Wistar rats were randomized into normal group,model group and total alkaloidal of tongbiling group(TBL group).TBL was extracted in Phytochemistry Room of Tropical Disease Institute of Guangzhou University of Traditional Chinese Medicine.METHODS:The animal model was prepared on rats with collagen induced arthritis.After treated with gastric infusion in groups, the pathological sections were done on phalangeal joint of rat and chondropathy was observed after routine HE staining.The chondrocytes were cultured.The chondrocytes were stimulated with rrIL 1β in vitro and resulted in matrix decomposition of chondrycyte to prepare pharmaceutical model,TBL of various concentrations added and dexamethasone(DXM) was applied in the control.After 48 hours culture,the upper clear solution if cell was collected and glycosaminoglycan(GAG) content was measured with alcian blue method, and nitric oxygen(NO) content was assayed with method of aqua fortis reduction.MAIN OUTCOME MEASURES:① Primary results:Effect of TBL on phalangeal chondropathy in rats with collagen induced arthritis and on matrix decomposition of chondrocyte induced by rrIL 1β .② Secondary results:Effect of TBL on induction of NO synthesis from chondrocytes by rrIL 1β .RESULTS:① It was indicated in evaluation on HE pathological section of phalangeal joint:The severity of cartilage destruction in TBL group was less than that in model group(P< 0.05).② TBL of various concentrations and DXM inhibited remarkably the decomposition of choncrocyte proteoglycan(P< 0.01) and NO synthesis (P< 0.01).CONCLUSION:TBL inhibits phalangeal cartilage destruction of rats with collagen induced arthritis.It is indicated in the results of experiment in vitro that TBL is against the decomposition of choncrocyte proteoglycan,which is probably achieved by inhibiting NO synthesis.更多还原