【摘要】 背景:滨蒿内酯是传统中药茵陈的主要有效成分之一,具有免疫抑制、消炎和舒张支气管平滑肌的作用。滨蒿内酯是否能改善哮喘豚鼠血清及肺组织匀浆中白细胞介素4与干扰素γ(IL-4/IFN-γ)失衡、进而抑制IgE的产生而发挥平喘作用目前尚不清楚。目的:研究滨蒿内酯的平喘作用及其对哮喘豚鼠免疫功能的调节作用。设计:以实验动物为研究对象,随机对照的验证性研究。单位:一所大学的天然药物研究室。材料:实验于2000-07/2002-03在中国医科大学药理教研室完成。选择健康雄性豚鼠84只,体质量200±50)g,清洁级。(干预:分别采用10g/L组胺与1g/L乙酰胆碱混合液引喘及10mL/L卵蛋白致敏复制法制备急性和迟发性哮喘豚鼠模型,采用雾化吸入途径给予滨蒿内酯药治疗,观察引喘潜伏期,并分别采用化学发光法、放免法和双抗夹心法检测迟发性哮喘豚鼠血清及肺组织匀浆中IgE,IL-4和IFN-γ的含量。主要观察指标:引喘潜伏期以及实验动物血清及肺组织匀浆中IgE,IL-4和IFN-γ含量。结果:滨蒿内酯明显延长急性和迟发性哮喘引喘潜伏期,哮喘组豚鼠血清及肺组织匀浆中IgE,IL-4含量较对照组明显增加(P<0.05),IFN-γ含量明显降低(P<0.05);而滨蒿内酯组IgE水平及IL-4含量较哮喘组豚鼠显著降低(P<0.05),IFN-γ的含量则明显升高(P<0.05)。结论:滨?更多还原

【Abstract】 BACKGROUND:Scoparone,which is a major principle separated from traditional Chinese herbs artemisia scoparia,which possesses actions of immunosuppression,anti inflammation and relaxing bronchial smooth muscle.It’s not known whether scoparone possesses the regulation of the imbalance of interferon gamma(IFN γ ) and interleukin 4(IL 4) in asthmatic guinea pig. OBJECTIVE:To investigate the inhibition and immunoregulation of scoparone on asthmatic guinea pig. DESIGN:Randomized controlled study based on the experimental animals. SETTING:Department of ethnopharmacology in a university.MATERIALS:The experiment was completed in the Department of Ethnopharmacology,China Medical University between July 2000 and March 2002.Totally 84 male guinea pigs were selected at sanitation grade,weighting about(200± 50) g.INTERVENTIONS:The ameliorative effects of scoparone on the imbalance of IFN γ and IL 4 in asthmatic guinea pigs were investigated by using the acute and delayed asthmatic guinea pigs which were respectively challenged by the aerosol mixture of 10 g/L histamine and 1 g/L acetylcholine,and 10 mL/L ovalbumin. MAIN OUTCOME MEASURES:The asthmatic latent periods in acute and delayed asthma,and the levels of IgE,IL 4 and IFN γ in serum and pulmonary tissue homogenate in the asthmatic animals. RESULTS:Scoparone significantly prolonged the asthmatic latent periods in acute and delayed asthma,observably reduced the levels of IgE and IL 4 and raised the content of IFN γ in serum and pulmonary tissue homogenate in the asthmatic animals(P< 0.05). CONCLUTION:The anti asthmatic effects of scoparone result from the ameliorative effects on the imbalance of IFN γ /IL 4 ratio thereby to inhibit the abnormal synthesis of IgE.更多还原