【摘要】 背景:慢性颈脊髓压迫症的发病机制和病理生理变化没有完全阐明。目的:建立兔慢性颈脊髓腹侧压迫模型,探讨脊髓慢性压迫后的病理和电生理的改变。设计:以实验动物为研究对象,随机对照的观察性研究。单位:一所大学医院动物实验中心和一所军医大学医院骨科实验室。材料:实验于2002-06/2003-04在南通医学院动物实验中心和上海长征医院骨科实验室完成。12周龄健康中国大白兔60只,雌雄不限,体质量2.5～3.0kg。随机分为正常对照组6只,实验组54只。方法:经颈前入路C5椎体置入钛合金螺钉,进行渐进性加压,建立慢性颈脊髓压迫模型。主要观察指标:主要结局:①组织学检查。②电生理检查。次要结局:神经功能评分。结果:48只白兔进入结果分析,其中正常对照组6只,实验组42只。实验组有31只出现脊髓病症状,改良Tarlov运动功能评分均为3分;其他11只无脊髓病症状评分为4分。正常对照组动物、实验组未出现脊髓压迫症状动物和实验组中出现慢性脊髓压迫症状动物的CSEPN1波潜伏期分别为(9.11±1.61),(11.36±2.17),(17.55±3.73)ms。实验组中出现慢性脊髓压迫症状的动物的皮质体感诱发电位(CSEP)N1波,与正常对照组和实验组未出现脊髓压迫症状的动物相比,潜伏期明显延长,差异有显著性意义(P<0.05)。结论:本模型可模拟临床慢性颈?更多还原

【Abstract】 BACKGROUND:Pathogenesis and pathophysiological changes of chronic compressive cervical myelopathy have not been completely clarified. OBJECTIVE:To establish an animal model of experimental chronic compressive cervical myelopathy for the exploration of the pathological and electrophysiological changes after chronic spinal compression. DESIGN:A randomized and controlled observatory study using experimental animals as subjects. SETTING:An Animal Experimental Center of a University and an Orthopaedic Laboratory of Affiliated Hospital of a Military Medical University MATERIALS:The study was conducted in the Animal Experimental Center of Nantong Medical University and the Orthopaedic Laboratory of Shanghai Changzheng Hospital from June 2002 to April 2003. Sixty 12 week healthy Chinese rabbits of either gender with a bodymass between 2.5 kg and 3.0 kg were randomly divided into control group(n=6) and study group(n=54). METHODS:Titanic metal screw was put into C5 vertebra through cervical anterior approach for progressive compression to establish chronic cervical myelopathy model. MAIN OUTCOME MEASURES:Principal consequences:① histological examination;② electrophysiological examination. Secondary consequence:neural function evaluation. RESULTS:Totally 48 rabbits entered into result analysis,in which 6 rabbits from control group and 42 rabbits from study group.Modified Tarlov’s motor function evaluation was 3 in 31 rabbits with compression signs,and 4 in 11 rabbits without compression signs.The latency of N1 wave in cortical somatosensory evoked potentials (CSEP) was(9.11± 1.61),(11.36± 2.17) and(17.55± 3.73) ms respectively in animals of control group,animals of study group without compression signs and animals of study group with compression signs.The lantency of CSEP N1 wave was significantly longer in animals of study group with compression signs than that of the animals in the control group and study group without compression signs(P< 0.05). CONCLUSION:This animal model can simulate clinical invasion process of chronic compressive cervical myelopathy.The severer the spinal compression is,the more often the compression signs appear, the longer the lantency of CSEP N1 wave is,and the more serious the spinal pathological damages are.更多还原