【摘要】 研究了有机溶剂中脂肪酶催化合成维生素A酯。对催化合成维生素A棕榈酸酯反应的脂肪酶和反应介质进行了比较,同时对影响合成维生素A棕榈酸酯反应的因素(温度、初始水含量、底物摩尔比、反应时间和酶量等)进行了探讨,优化了反应条件:在10mL不含水分的正己烷中,0.200g维生素A醋酸酯和0.468g棕榈酸在酶量为10%(指固定化酶与维生素A醋酸酯的质量比)的固定化脂肪酶催化下,在30℃、190r/min下反应6h,转化率可以达到75%,固定化酶可连续使用6次以上。更多还原

【Abstract】 The synthesis of vitamin A esters in organic solvent with a lipase was studied.Novozym 435 was used for the synthesis of vitamin A plamitate.The influences of solvent,the molar ratio of substrates,the reaction temperature and time,and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid,at 30℃,in 10 mL hexane,containing 0% of water(V/V),with 10% of lipase(mass ratio,immobilized lipase to vitamin A plamitate).In these conditions,75% of vitamin A acetate was converted into vitamin A plamitate.The immobilized lipase was reused about 6 batches.更多还原