【摘要】 目的:研究艾灸“肺俞”“膏肓”对博莱霉素(BLM)所致肺纤维化大鼠肺组织Ⅰ/Ⅱ型细胞因子平衡调节的影响,探索针灸阻抑肺纤维化的效应机制。方法:SD大鼠随机分为:空白组、模型组、艾灸组、泼尼松组。气管内注入博莱霉素制作大鼠肺纤维化模型,造模后7 d开始治疗,以5 mg左右艾绒灸其双侧“肺俞”“膏肓”,每穴3壮,每天1次,10 d为1疗程,疗程间休息1 d,3个疗程后处死动物,采用酶联免疫吸附法测定肺组织内Ⅰ型细胞因子IL-2I、L-12和Ⅱ型细胞因子IL-4、IL-5含量。结果:造模后大鼠肺组织内Ⅰ型细胞因子含量降低,Ⅱ型细胞因子含量升高(均P<0.01);艾灸及泼尼松治疗能够使大鼠肺组织内Ⅰ型细胞因子含量升高,Ⅱ型细胞因子含量降低,与模型组比较具有统计学差异(P<0.05,0.01);艾灸组与泼尼松组比较,各指标均无统计学差异(P>0.05)。结论:艾灸“肺俞”“膏肓”能升高BLMA5诱导肺纤维化大鼠肺组织中Ⅰ型细胞因子IL-2I、L-12的含量,降低Ⅱ型细胞因子IL-4I、L-5的含量,提示调节Ⅰ/Ⅱ型细胞因子的平衡可能是艾灸阻抑肺纤维化发生的机理之一。更多还原

【Abstract】 Objectives: To observe the effect of moxibustion of "Feishu"（BL 13） and "Gaohuangshu"（BL 43） on the balance of T helper1/T helper2（Th1/Th2） cytokines in BLM_A5-induced pulmonary fibrosis rats,and to explore its underlying mechanism.Methods: Twenty-four SD rats were evenly randomized into control,model,moxibustion,and medication groups.Pulmonary fibrosis model was established by injection of Bleomycin via trachea（5 mg/kg）.Moxibustion（3 cones/acupoint） was applied to bilateral "Feishu"（BL 13） and "Gaohuangshu"（BL 43）,once daily,with 10 days being a therapeutic course and an interval of one day,3 courses altogether.Rats of medication group were fed with Prednisone（2.5 mg/kg）,once daily and continuously for 30 days.At the end of experiments,the rats were killed for taking out the left mid-lung tissues to be processed into homogenate samples.Cytokines such as IL-2,IL-12,IL-4 and IL-5 were detected with enzyme linked immunosorbent assay according to instructions of reagent kits.Results: In comparison with control group,IL-2 and IL-12 contents of the lung tissue were significantly lower,while IL-4 and IL-5 considerably higher in model group（P<0.01）;whereas compared with model group,IL-2 and IL-12 contents were significantly higher（P<0.05,0.01）,IL-4 and IL-5 levels significantly lower in moxibustion and medication group（P<0.01）.No significant differences were found between moxibustion group and medication group in the 4 indexes（P>0.05）.It indicated that in BLM_A5-induced pulmonary fibrosis rats,Th1 cytokines IL-2 and IL-12 levels in the lung tissue decreased markedly,and Th2 cytokines IL-4 and IL-5 levels increased abnormally, both moxibustion and Prednisone could regulate changes of Th1 and Th2 cytokines.Conclusion: Moxibustion of "Feishu"（BL 13） and "Gaohuangshu"（BL 43） can raise the contents of Th1 cytokines（IL-2 and IL-12） and lower those of Th2 cytokines（IL-4 and IL-5）,suggesting that moxibustion induced balance between Th1 and Th2 cytokines may contribute to its action in resisting pulmonary fibrosis.更多还原