【摘要】 探讨了碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)能否对人结膜下Tenon’s囊成纤维细胞(humansubconjunctivalTenon’scapsulefibroblast,HTCF)表型改变起诱导作用。在5例白内障手术中取人结膜下Tenon’s囊组织块培养成纤维细胞。用不同浓度bFGF(0、5、10、20ng/ml)诱导HTCF48h。免疫细胞化学技术、蛋白质印记免疫技术检测其平滑肌肌动蛋白(α-smoothmuscleactin,α-SMA)表达与正常HTCF有否差异。实验结果表明用不同浓度(0￣20ng/ml)bFGF诱导HTCF48h,虽能明显促进HTCF细胞的生长,但均不能上调细胞内α-SMA的表达。在0￣20ng/ml范围内,体外用bFGF诱导HTCF48h,不能诱导其改变为肌成纤维细胞。更多还原

【Abstract】 The purpose of the present study was to explore whether human basic fibroblast growth factor (bFGF) had the effect on phenotypic transition of human subconjunctival Tenon’s capsular fibroblast (HTCF) after operation or trauma. Cultured HTCF derived from 5 human cataract operation cases were induced by bFGF of different concentration (0, 5, 10, 20 ng/ml) for 48 h. Then α-smooth muscle actin (α-SMA) was detected by immunocytochemistry and Western blot procedure, and it could be observed whether there was differential expres- sion in comparison with normal HTCF. Cultured HTCF stimulated with different concentration of bFGF for 48 h induced that bFGF could not up-regulate the expression of α-SMA. bFGF from 0–20 ng/ml for 48 h could not induce HTCF transit to myofibroblast in vivo.更多还原