【摘要】 骨髓间充质干细胞(BMSC)和神经前体细胞(NPC)移植于脑组织损伤动物的实验证明这两类细胞移植后均能在体内迁徙,与周围细胞整合,促进神经功能修复。BMSC促进神经功能修复的机制之一被认为与其分泌一些细胞因子和趋化因子有关,但具体机制不十分明确。为从基质细胞衍生因子-1α(SDF-1α)及其唯一的受体CXCR4这对分子相互作用的机制上探讨BMSC移植的可能治疗作用,实验采用ELISA法检测了体外培养的BMSC上清液中SDF-1α的含量,体外微孔隔离室迁移实验发现NPC能在BMSC分泌的培养上清液中SDF-1α的作用下发生定向迁移,特异性抗CXCR4单抗能有效阻断NPC的定向迁移效应,证实了BMSC分泌的SDF-1α促进表达CXCR4的NPC向病灶处迁移可能是促进神经功能修复的机制之一,从而为干细胞移植治疗神经功能缺损提供了一个新的思路。更多还原

【Abstract】 Brain trauma examination indicated that both bone marrow mesenchymal stem cells (BMSC) and neural progenitor cells (NPC) could migrate after transplantation, in vivo, and integrated into the cells around of them, so as to accelerate the recovery of neurological impairment. Although the mechanism of it is not clear, it was speculated that some chemokines or cytokines secreted by BMSC might contribute to it. This study discussed the possible mechanism dealing with the molecular SDF-1α/CXCR4. BMSC were confirmed by ELISA to secret high level of SDF-1α [about (1860.00±526.29) pg/ml]. Suggested by Boyden Chamber Assay, BMSC were able to chemoattract the CXCR4 positive NPC migration in vitro, which could be neutralized by the monoclonal antibody CXCR4. It is speculated that TNF-α may up-regulate the expression of CXCR4 on the NPC, thus enhance the migration ability of NPC. This provided a novel idea to neurological impairment therapy by stem cells transplantation.更多还原