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DH群体内株系对籼、粳亚种的亲和性

Compatibility of DH (Doubled Haploid) Lines from Indica/Japonica Hybrid to Indica and Japonica

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【作者】 张再君梁承邺朱英国

【Author】 ZHANG Zai-Jun 1,3, LIANG Cheng-Ye2, ZHU Ying-Guo1 (1 The Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072, Hubei; 2 South China Institute of Botany, Chinese Academy of Sciences.Guangzhou, 510650, Guangdong; 3Hubei Agricultural College, Jingzhou 434025, Hubei,China)

【机构】 武汉大学生命科学院植物发育生物学教育部重点实验室中国科学院华南植物研究所武汉大学生命科学院植物发育生物学教育部重点实验室 湖北武汉430072湖北农学院湖北荆州434025广东广州510650湖北武汉430072

【摘要】 以窄叶青8号(ZYQ8) 京系17(JX17)的13个花培DH株系和培矮6 4 / 0 2 4 2 8的11个花培DH株系为材料,分析了它们的籼粳分化及其对籼、粳亚种杂交的亲和性。结果表明,无论亲本是否具有广亲和性亲缘,两个DH群体均出现广亲和性良好的株系,且多数株系属籼粳中间类型。这说明虽然广亲和性是遗传的,但是籼粳基因的重组确实可以产生广亲和性,而且籼粳中间型可能是广亲和性表达的最适遗传背景。对农艺性状的分析认为,DH株系的广亲和性与株系本身的自交结实率和脱粒性没有联系。通过窄叶青8号 京系17DH株系对细胞质雄性不育恢复性的分析表明,雄性不育可恢复性与广亲和性是相互独立的。

【Abstract】 The total 24 accessions selected randomly from two DH (doubled haploid) populations, which were derived from inter-subspecific crosses Zhaiyeqing 8 / Jingxi 17 and Pei’ai 64 / 02428 respectively, were crossed with four wide compatibility tester varieties in order to investigate their wide compatibility. According to Virmani S S(1992), a DH line tested will be designated as wide compatible (WC) line, if the spikelet fertility of its tested hybrid F 1 is significantly higher than that of the indica/japonica F 1 but similar to indica and japonica tester parents. A t test was used to compare the significance of differences in mean spikelet fertilities of the four testcrosses for each DH lines and the control crosses and the parents. The results (Table 2) indicated that DH lines with wide compatibility appeared in the two DH populations, no matter whether their parents had wide compatibility or not. This suggested that the recombination between indica and japonica genes could lead to wide compatibility. Interestingly, the wide compatibility of DH line had no correlation with self-pollination seed-setting percentage and seed threshing (Table 3). Moreover, most of the DH lines with wide compatibility belonged to an intermediate type between indica and japonica identified by means of Cheng’s morphological traits index method (1987). In addition, the restoring fertility ability to cytoplasmic male sterile was independent of wide compatibility in the DH lines.

【基金】 湖北省教育厅重点科技项目资助 (2 0 0 0B0 5 0 0 6);湖北省科技厅十五育种攻关项目资助 (2 0 0 1AA2 0 4A0 1)
  • 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,2005年04期
  • 【分类号】S511.2
  • 【被引频次】2
  • 【下载频次】110
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