【摘要】 分析了2 2 1份春小麦品种(系)的HMW GS、LMW GS组成和1BL 1RS易位状况,并用其中10 4份品种(系)研究了HMW GS和LMW GS等位变异及1BL 1RS易位对品质性状的影响。结果表明,1、7+9、5 +10、GluA3a和GluB3j分布较广,频率分别为5 7 .5 %、4 5 . 2 %、6 3 .8%、2 9. 0 %和4 2 . 5 %。1BL- 1RS易位系相当普遍,西北春麦区和东北春麦区频率分别为4 4 . 3%和34 2 %。HMW- GS和LMW -GS等位变异对SDS沉降值、和面时间与耐揉性的影响达1%显著水平,对籽粒蛋白质含量与不溶性谷蛋白含量的影响达5 %显著水平。按位点贡献大小,Glu- D1>Glu- B3>Glu -B1>Glu- A1>Glu -A3;不同亚基对品质性状的效应存在显著差异,主要表现在反映面筋强度的品质参数上。亚基1、2 、5 +10、Glu -A3d、Glu- B3f明显优于相应位点的其他亚基。1BL -1RS易位对和面时间有极显著的负向效应。更多还原

【Abstract】 The composition of high molecular weight glutenin subunits (HMW-GS) and low molecular weight glutenin subunits (LMW-GS), and the presence or absence of 1BL/1RS translocation play an important role in determination of wheat quality. Totally, 221 spring wheat cultivars and advanced lines were used to survey the composition of HMW-GS and LMW-GS, and the presence of 1BL/1RS translocation. Effects of allelic variation in HMW-GS and LMW-GS and 1BL/1RS translocation on quality traits were investigated in 104 cultivars and lines. The results showed that subunits 1, 7+9, 5+10, GluA3a and GluB3j took dominant positions with frequencies of 57.5%, 45.2%, 63.8%, 29.0% and 42.5%, respectively, and 44.3% of tested genotypes from Northwestern Spring Wheat Region (NWSWR) and 34.2% from Northeastern Spring Wheat Region (NESWR) carried 1B/1R translocation (Table 1). Allelic variation at Glu-1 and Glu-3 loci had significant influence on SDS sedimentation volume, and mixing time and mixing tolerance at 1% probability level, and on kernel protein content and relative insoluble glutenin content at 5% significant level (Table 2). According to the allele contribution to mixing time and mixing tolerance, the glutenin subunit loci were ranked as: Glu-D1> Glu-B3> Glu-B1> Glu-A1> Glu-A3 (Table 3). Various individual glutenin alleles had significant effect on quality traits, specially on gluten strengthen related parameters. They were ranked as: at Glu-A1 loci,1,2* >N, at Glu-D1,5+10 > 2+12, at Glu-A3,Glu-A3d>Glu-A3b>Glu-A3c>Glu-A3a,at Glu-B3,Glu-B3f>Glu-B3b>Glu-B3j (Table 3). The contribution of subunits 1, 2*, 5+10, Glu-A3d, Glu-B3f to mixing time and mixing tolerance are significantly larger than their counterpart allelic variations. The presence of 1B/1R translocation had a strong and negative effect on mixing time. The mixing time of non-1BL/1RS cultivars and lines was 3.9 min while 1BL/1RS translocation was 3.5 min (Table 4).更多还原