【摘要】 小麦EST数量的迅速增加为开发新的SSR标记提供了宝贵的数据资源 ,本实验从国际小麦族EST协作网 (ITEC)上公布的 10 380条EST序列中检索到 4 4 4条含有SSR的序列 ,检出率为 4 1%。其中含二核苷酸重复单元和三核苷酸重复单元的SSR ESTs分别为 34条 (7 7% )和 347条 (78 0 % )。利用这些SSR ESTs序列共设计 135对cSSR引物 ,其中 82对在小麦上有扩增产物 ,占所设计引物总数的 6 0 8%。利用小麦缺体 四体 ,32对cSSR引物被定位到小麦除 2D、4B和 4D外的 18条染色体上。更多还原

【Abstract】 The growing availability of EST sequences from wheat provides a potential valuable source of new SSR markers. In this study, 444 SSR-ESTs from 10 380 ESTs in the International Triticeae EST Cooperative (ITEC) database, representing 4.1% of the total number of ESTs were identified. Among them, 34 dinucleotide repeats and 347 trinucleotide repeats were found, accounting for 7.7% and 78.0% of the total SSR-ESTs respectively. 135 cSSR primers were designed to sequence flanking SSRs from 175 selected SSR-ESTs, of which 82 primers were efficient in wheat. 32 of the 82 cSSR primers were located on 18 wheat chromosomes except for 2D, 4B and 4D of 21 wheat chromosomes.更多还原