【摘要】 多酚氧化酶 (polyphenoloxidase ,PPO)是引起面团 (片 )颜色褐变的主要原因。利用 12 2对SSR引物、4对贮藏蛋白STS引物和 10对AFLP引物组合 ,分析了中优 95 0 7×CA96 32的 71个DH系的基因型 ,构建了由 173个位点组成的遗传连锁图 ,在小麦 2 1个连锁群上覆盖 2 881cM。将该群体种植于 3个环境 ,采用复合区间作图法 (CIM)进行了PPO活性的QTL分析。结果表明 ,控制籽粒PPO活性的主效QTL有 2个 ,分别位于染色体 2AL和 2DL上 ,贡献率分别为 37 9%～5 0 0 %和 2 5 0 %～ 2 9 1%。所有QTL都来自高PPO活性亲本中优 95 0 7。讨论了通过遗传途径降低PPO活性及利用分子标记辅助育种的可能性。更多还原

【Abstract】 The polyphenol oxidase (PPO) activity is a major factor to cause an undesirable brown discoloration of wheat-based end products during processing or storage.A double haploid (DH) population with 71 lines derived from Zhongyou 9507 with higher PPO activity and CA9632 with lower PPO activity, were sown at three locations with two replications.A total of 173 loci included SSR, STS of storage protein and AFLP markers were used to construct a linkage map, covering 2 881 cM of whole genome at 21 linkage groups.With the method of composite interval mapping (CIM), two putative major QTLs were detected on chromosome 2AL and 2DL, accounting for 37.9%-50.0% and 25%-29.1% of the phenotypic variance across three environments, respectively.The QTL on 2AL played a more significant role than the QTL on 2DL.Reduction of PPO activity through genetic improvement and molecular marker assisted selection was also discussed.更多还原