【摘要】 应用正交设计法研究了植物生长调节剂、硝酸银和活性炭等对七姊妹愈伤组织的诱导、不定芽诱导 和生根培养的影响。结果表明:最佳愈伤组织诱导培养基为MS+2．4-D 0．8mg／L+6-BA 0．2 mg／L+蔗糖 30g／L+琼脂7．0 g／L;不定芽诱导最佳培养基为MS+NAA 0．1mg／L+CPPU0．8 mg／L+AgNO34.0mg／L +蔗糖30g／L+琼脂7．0 g／L,分化率为95％,增殖率为5．5;最佳生根培养基为1／4MS+NAA 0．3 mg／L+ IBA 0．3 mg／L+蔗糖20g／L+琼脂4．0 g／L,生根率为90％。更多还原

【Abstract】 Using orthogonalty design L9(34),we studied the effect of plant growth regulators ,AgNO3 and activated carbon etc on the induction of callus , adventition buds induction and the adventition bud rooting. The best medium for inducing callus is MS + 2,4-D 0. 8mg/L + 6-BA 0. 2 mg/L +sucrose30 g/L+agar 7. 0 g/L; For adventition Buds, the medium is MS + NAA 0. 1mg/L +CPPU 0. 8 mg/L + + AgNO34. 0 mg/L + sucrose 30 g/L+ agar 7. 0 g/L, differentiation rate is 95%,the multiplication rate is 5. 5; As for the induction of rooting the only medium is 1/4MS+ NAA 0. 3 mg/L+ IBA 0. 3 mg/L+ sucrose 20 g/L + agar 4. 0 g/L,the rooting rate is更多还原