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唐棣和鞘柄木的组培及消毒技术研究

Studies on Tissue Culture and Sterilization of Amelanchier sinica and Torricellia angulata var. intermedia

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【作者】 高崇辉吉文丽张小波

【Author】 GAO Chong-hui~1, JI Wen-li~2, ZHANG Xiao-bo~3(1. Forest Bureau of Xining, Xining, Qinghai 810000, China; 2. College of Forestry, Northwest A & F University,Yangling,Shaanxi 712100, China;3. Northwest Forest Inventory and Planning Institute, The State Forestry Administration, Xi’an,Shaanxi 710048, China )

【机构】 青海省西宁市林业局西北农林科技大学林学院国家林业局西北林业调查规划设计院 青海西宁810000陕西杨陵712100陕西西安710048

【摘要】 以唐棣和角叶鞘柄木的叶器官作为外植体,以MS和B5作为基本培养基,采用不同浓度的IAA、NAA,2,4D和6BA,对唐棣和角叶鞘柄木的组培过程进行了研究。唐棣在MS培养基添加IAA0.5+NAA0.5+6BA2.0上愈伤组织生长较好;角叶鞘柄木则以B5培养基添加2,4D1.0+6BA0.5愈伤组织生长较好。

【Abstract】 The research was carried out upon the leaves explants of Amelanchier sinica and Torricellia angulata var. intermedia. The media were MS and B5 medium. Supplemented with different concentrations of IAA、NAA、2,4-D and 6-BA. Of Amelanchier sinica, callus were induced on MS medium, which is better than on B5 medium. Better medium and phytohormore combination was: MS+IAA 0.5+6-BA 2.0 mg·L-1; Of Torricellia angulata var. intermedia, better medium and phytohormone combination was: BM5+2,4D 0.5+6-BA 0.5 mg·L-1.

  • 【文献出处】 西北林学院学报 ,Journal of Northwest Forestry University , 编辑部邮箱 ,2005年02期
  • 【分类号】S792.99
  • 【被引频次】6
  • 【下载频次】133
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