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唐棣和鞘柄木的组培及消毒技术研究
Studies on Tissue Culture and Sterilization of Amelanchier sinica and Torricellia angulata var. intermedia
【摘要】 以唐棣和角叶鞘柄木的叶器官作为外植体,以MS和B5作为基本培养基,采用不同浓度的IAA、NAA,2,4D和6BA,对唐棣和角叶鞘柄木的组培过程进行了研究。唐棣在MS培养基添加IAA0.5+NAA0.5+6BA2.0上愈伤组织生长较好;角叶鞘柄木则以B5培养基添加2,4D1.0+6BA0.5愈伤组织生长较好。
【Abstract】 The research was carried out upon the leaves explants of Amelanchier sinica and Torricellia angulata var. intermedia. The media were MS and B5 medium. Supplemented with different concentrations of IAA、NAA、2,4-D and 6-BA. Of Amelanchier sinica, callus were induced on MS medium, which is better than on B5 medium. Better medium and phytohormore combination was: MS+IAA 0.5+6-BA 2.0 mg·L-1; Of Torricellia angulata var. intermedia, better medium and phytohormone combination was: BM5+2,4D 0.5+6-BA 0.5 mg·L-1.
【关键词】 唐棣;
鞘柄木;
外植体;
组培;
【Key words】 Amedlanchier sinice; Torricellia angulata var. intermedia; explants; tissue culture;
【Key words】 Amedlanchier sinice; Torricellia angulata var. intermedia; explants; tissue culture;
- 【文献出处】 西北林学院学报 ,Journal of Northwest Forestry University , 编辑部邮箱 ,2005年02期
- 【分类号】S792.99
- 【被引频次】6
- 【下载频次】133