【摘要】 目的:了解抗血管内皮生长因子(VEGF)中和抗体对Ⅱ型胶原(CoⅡ)诱导的关节炎(CIA)形成的影响。方法:于8~10wk龄的DBA/1J小鼠尾根部皮内注射鸡CoⅡ进行被动免疫,建立小鼠CIA模型,以CIA发生率、关节炎指数及关节组织的病理变化为指标,评价抗VEGF抗体对CIA形成的影响。结果:与对照组相比较,在CIA形成的早期,注射抗VEGF中和抗体能有效地抑制关节炎的产生及减轻其严重程度(P<0.05);而在CIA已形成后再注射抗VEGF中和抗体对关节炎的严重程度则无明显影响(P>0.05)。结论:抗VEGF中和抗体明显抑制滑膜细胞增殖、血管新生及血管炎,因此有望成为类风湿性关节炎(RA)治疗的新型制剂。更多还原

【Abstract】 AIM: To understand the effect of neutralizing anti-vascular endothelial growth factor (VEGF) antibody on the genesis of murine type Ⅱ collagen (COⅡ)-induced arthritis (CIA). METHODS: mice DBA/1J (8-10 weeks) were given intradermal injection chick tape Ⅱ collagen to set up the murine CIA model. The incidence of CIA and arthritis index were measured and the pathologic changes of articular tissue were observed after the injection of neutralizing anti-VEGF antibody. RESULTS: The anti-VEGF antibody could notably inhibit the genesis and severity of arthritis ((P<0.05)) at the early stage of CIA formation; while it made no difference after CIA was completely formed. CONCLUSION: Anti-VEGF neutralizing antibody can significantly inhibit synovial fibroblast proliferation, neovascularization and angeitis genesis, which made it a promising agent for RA treatment.更多还原