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甲胺磷农药对小白菜中几种抗氧化物的影响

The Impact of Methamidophos to the Activity of Antioxidant Compounds in Chinese Cabbage

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【作者】 熊丽生秀梅唐红枫王媛刘涛刘喜平栾勇成

【Author】 XIONG Li~(1), SHENG Xiu-Mei~(1,2), TANG Hong-Feng~1, WANG Yuan~1, LIU Tao~1, LIU Xi-Ping~1, LUAN Yong-Cheng~1(1.College of Life Science, Central China Normal University, Wuhan 430079, China; 2.Department of Biochemistry, Jiangsu University, Zhenjiang, Jiangsu 212001, China)

【机构】 华中师范大学生命科学学院华中师范大学生命科学学院 武汉430079武汉430079江苏大学医学技术学院生化教研室江苏镇江212001武汉430079

【摘要】 以小白菜为研究对象,喷洒一定浓度甲胺磷农药后,测定7d内小白菜体内可溶性蛋白质的含量及各种抗氧化物如超氧化物歧化酶(superoxidedismutase,SOD)、谷胱甘肽(glutathione,GSH)、谷胱甘肽-S-转移酶(glu-tathione-S-transferase,GST)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathioneperoxidase,GPX)等活性的变化。结果表明,喷药后第1天各种物质含量相对与对照变化不大,第2~6天小白菜中蛋白质、GSH、SOD、CAT都高于对照,而GPX、GST的含量低于对照,第7天都又还原到与对照接近。说明有机磷农药喷洒后植物体内产生了氧自由基,进而诱导细胞内防御活性氧自由基毒害的物质产生,5~6天后机体恢复正常。实验为食品卫生检验提供了一个参考标准。

【Abstract】 In this paper, the content of soluble protein and the activity of SOD, GSH, GST, CAT, GPX in Chinese cabbage were determined in the following 7 days after sprayed by 0.5 g/m~2 Methamidophos. The results indicated that the content of all parameters didn’t change obviously in the first day. In the 2-6 day, the content of protein, and the activity of GSH, SOD and CAT in Chinese cabbage were higher than the control, but the content of GPX and GST were relatively lower. At the 7th day, all parameters revived to the level of control sample. The results demonstrated that treated by Methamidophos, the antioxidant compounds of Chinese cabbage were induced to withstand the toxicity of Methamidophos.

【关键词】 甲胺磷农药小白菜蛋白质GSHSODGSTGPXCAT
【Key words】 MethamidophosChinese cabbageProteinGSHSODGSTGPXCAT
【基金】 武汉市青年科技晨光项目(2002500103);科技部基础研究重大项目前期研究专项(2001CCB00700)。
  • 【文献出处】 武汉植物学研究 ,Journal of Wuhan Botanical Research , 编辑部邮箱 ,2005年04期
  • 【分类号】X173
  • 【被引频次】25
  • 【下载频次】184
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