【摘要】 建立一种蒽酮-硫酸法测定西洋参多糖质量分数的方法,并对不同西洋参中多糖的质量分数进行测定比较.西洋参用水提取,经多级处理除去脂类、单糖、双糖、低聚糖、氨基酸、蛋白质等杂质制得西洋参精制多糖,精制多糖溶解与蒽酮-硫酸试剂显色,通过扫描确定最大吸收波长.然后用精制多糖测得西洋参多糖对葡萄糖的换算因子,采用蒽酮-硫酸法比色测定.结果表明,此测定方法简便可行,供试液在4 h内显色稳定,重现性较好,平均回收率为96.9%,RSD=1.33%(n=4).来源于不同产地的西洋参多糖质量分数有一定的差异,其范围为6.32%~10.8%.更多还原

【Abstract】 An anthrone-H₂SO₄ colorimetry method was developed for the determination of polysaccharides in Panax quinquefolium L.samples from different origins.Polysaccharides in Panax quinquefolium.L samples were extracted with water,and the interference components such as lipid,monosaccharide,disaccharide,oligosaccharide,amino acids and protein were removed during the pretreatment process.The contents of the polysaccharides were determined by anthrone-H₂SO₄ colorimetry method,and a corrected factor was used in order to minimize the error of determination.The results showed that the method used was simple and practical,and the color of the treated samples could be stabilized within 4h.The average recovery rate for the polysaccharides was 96.6% with 1.33% of RSD（n=4）.The contents of polysaccharides in Panax quinquefolium.L from different origins ranged from 6.32% to 10.8%.更多还原