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生物素和VB1在谷氨酸棒杆菌过量合成缬氨酸中的作用

Effects of D-Biotin and VB1 Factors on the Biosynthesis of Valine in a Valine Producer Corynebacterium glutamicum AATV341

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【作者】 杨毅章志兰姜绪林杜大庆张星元

【Author】 YANG Yi, ZHANG Zhi-lan, JIANG Xu-lin, DU Da-qing, ZHANG Xing-yuan(The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036,China)

【机构】 江南大学工业生物技术教育部重点实验室江南大学工业生物技术教育部重点实验室 江苏无锡214036江苏无锡214036江苏无锡214036

【摘要】 通过对谷氨酸棒杆菌突变株AATV341进行生物素和VB1添加量的实验,探讨了生物素和VB1添加量对该菌株菌体质量及缬氨酸产量的影响;同时根据对AATV341进行的胞内外某些特定氨基酸和有机酸检测,研究了这两种维生素对碳架代谢流的分布,尤其是对丙酮酸节点处的代谢流量分布的影响.结果表明,生物素添加量在40μg/L时,菌体质量和缬氨酸在胞外的累积明显提高,谷氨酸在胞外累积降低;VB1的适量添加,可提高缬氨酸在胞外的累积,降低发酵液中乙酸和草酰乙酸的含量.

【Abstract】 In this paper, the effects of D-biotin and VB1 on overproduction of valine were studied, and the composition of culture medium was also optimized. The concentrations of several metabolites produced in batch cultire (extra-cellular and intra-cellular) were determined. The distributions of carbon-flux, especially at the node pyruvate were calculated, compared, and analyzed. The results showed that,in Corynebacterium glutamicum AATV341 cultivation, adding 40 (μg/L) biotin and 400 μg/L VB1 would increase the productivity of valine, and repress the extra-cellular concentrations of oxaloacetic acid, acetic acid and glutamate. The analysis may play an important role in re-designing metabolism to achieve further fermentative improvement.

  • 【文献出处】 食品与生物技术学报 ,Journal of Wuxi University of Light Industry , 编辑部邮箱 ,2005年04期
  • 【分类号】Q936
  • 【被引频次】24
  • 【下载频次】354
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