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生物素和VB1在谷氨酸棒杆菌过量合成缬氨酸中的作用
Effects of D-Biotin and VB1 Factors on the Biosynthesis of Valine in a Valine Producer Corynebacterium glutamicum AATV341
【摘要】 通过对谷氨酸棒杆菌突变株AATV341进行生物素和VB1添加量的实验,探讨了生物素和VB1添加量对该菌株菌体质量及缬氨酸产量的影响;同时根据对AATV341进行的胞内外某些特定氨基酸和有机酸检测,研究了这两种维生素对碳架代谢流的分布,尤其是对丙酮酸节点处的代谢流量分布的影响.结果表明,生物素添加量在40μg/L时,菌体质量和缬氨酸在胞外的累积明显提高,谷氨酸在胞外累积降低;VB1的适量添加,可提高缬氨酸在胞外的累积,降低发酵液中乙酸和草酰乙酸的含量.
【Abstract】 In this paper, the effects of D-biotin and VB1 on overproduction of valine were studied, and the composition of culture medium was also optimized. The concentrations of several metabolites produced in batch cultire (extra-cellular and intra-cellular) were determined. The distributions of carbon-flux, especially at the node pyruvate were calculated, compared, and analyzed. The results showed that,in Corynebacterium glutamicum AATV341 cultivation, adding 40 (μg/L) biotin and 400 μg/L VB1 would increase the productivity of valine, and repress the extra-cellular concentrations of oxaloacetic acid, acetic acid and glutamate. The analysis may play an important role in re-designing metabolism to achieve further fermentative improvement.
【Key words】 valine; Corynebacterium glutamicum; biotin; VB1; carbon flux; acetic acid; oxaloacetic acid;
- 【文献出处】 食品与生物技术学报 ,Journal of Wuxi University of Light Industry , 编辑部邮箱 ,2005年04期
- 【分类号】Q936
- 【被引频次】24
- 【下载频次】354